2011-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/672494摘要：背景及目的：本研究利用我們自行開發的作為溫熱治療的基本材料，這種具有磁性的粉末在交流電感應電場下可以提高溫度，藉由其帶有磁性且在磁場下可升溫的特性，並將其應用在癌症治療的溫熱療法上。方法及實施進度:計畫第一年（2010）:對材料進行生物相容性測試，為檢測材料是否對細胞造成毒性影響，將進行LDH 細胞毒性測試，此外，也利用MTT 對細胞增生做測試。在確認細胞相容性之後開始進行體外測試，測量在外加磁場下的腫瘤細胞以及正常細胞對溫熱治療的反應。嘗試不同的參數，以找出最適合的磁場強度、交流電頻率。第二年（2011）：進行一個月（subacute toxicity）及三個月（chronic toxicity）之體內生物相容性測試。建立癌症老鼠之動物模式，追蹤腫瘤大小，以實際瞭解我們的材料在生物體內治療癌症的效果。第三年（2012）：根據磁場強度、熱療頻率再去做更細的分類，以建立劑量—療效之曲線。目的在盡量減少熱治療之頻率。另將實驗動物模式繼續擴展成「骨骼癌症轉移」模式。預期研究及其可能應用:未來若能將表面改質，使之針對特定的腫瘤抗原，再經靜脈注射入人體內，則可以達到只殺死癌細胞的目的，藉此提供溫熱療法更有效且無害人體的新選擇。<br> Abstract: Objective, background and rationale:Hyperthermia is one of the most popular research topics for cancer therapy. This researchis aimed to develop a biodegradable magnetic nanoparticles based on Dicalcium PhosphateDihydrate (DCPD) for cancer hyperthermia. After applying external magnetic field by ACcurrent, we can raise the temperature of the material. Therefore the material is a possiblecandidate for cancer hyperthermia therapy.Material and Methods :First Year(2010): We will perform the cytotoxicity tests, including MTT and LDH test.We’ll explore whether the cell adhesion, proliferation and differentiation being affected by thematerial itself without external magnetic field. The in vitro test with external magnetic fieldwill be carried out. The thermal sensitivity of normal cells and cancer cells were measured.Second Year(2011): We will perform in vivo biocompatibility test for one month(subacute toxicity) and three months (chronic toxicity). We will establish an animal model andtest the magnetic DCPD particles on mice with cancer.Third Year(2012): We will further classify the mice into different groups, in terms of thestrength of magnetic field, the frequency of hyperthermia therapy and aim to reduce thefrequency of therapy. We will further establish another animal model of bone metastasis.Future work and possible significance:In the future, we can modify the DCPD with surface marker which aimed to specificcancer cells. It can be a new choice for hyperthermia to kill tumor cell more efficiently andwithout harming the normal human cells which may revolutionize cancer therapy on human.雙水磷酸氫鈣奈米粒子癌症磁滯損失癌症熱治療sol-gel methodbioglassbiodegradablehysteresis losshyperthermia