Chen, Jr WeiJr WeiChenLin, Yi LingYi LingLinSamuel Wu, Yi HsiengYi HsiengSamuel WuSHENG-YAO WANGCHUNG-HSI CHOUYI-CHEN CHEN2023-04-172023-04-172021-04-0100325791https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101595301&doi=10.1016%2fj.psj.2021.01.031&partnerID=40&md5=15f20b65f3b9d843bda6525319fcc5e6https://scholars.lib.ntu.edu.tw/handle/123456789/630234Approximately 400 metric tons of egg chalazae, a byproduct in the liquid-egg processing plant, are produced yearly but always regarded as a waste in Taiwan. Our team successfully developed a crude egg chalaza hydrolysate by protease-A digestion (CCH-A). Free branched-chain amino acids, 3-aminoisobutyric acid, and β-alanine, and anserine were assayed in the CCH-A used in this study. Besides, the in vitro bile-acid binding ability and inhibitory lipase activity of CCH-As were demonstrated. Then, high-fat diet feeding for 10 wk caused hyperlipidemia, insulin resistance, and hepatosteatosis in hamsters (P < 0.05). However, CCH-A co-treatment decreased serum/liver triglyceride levels and lipid accumulation in livers by increasing daily fecal lipid/bile-acid outputs, upregulating fatty-acid β oxidation, and downregulating fatty-acid biosynthesis in livers (P < 0.05). CCH-A co-treatment also amended insulin resistance, augmented hepatic antioxidant capacity, and decreased liver damages and inflammatory responses (P < 0.05). Taken together, our results do not only demonstrate the hepatoprotective effects of CCH-As against a chronic high-fat dietary habit, achieving effects similar to Simvastatin, but also decrease the environmental burden of handling chalazae in the liquid-egg industry.encrude-chalaza hydrolysate | hepatosteatosis | high-fat diet | insulin resistance | lipid homeostasis[SDGs]SDG3[SDGs]SDG9[SDGs]SDG11adverse event; animal; chicken; fatty liver; hamster; insulin resistance; lipid diet; lipid metabolism; liver; metabolism; rodent disease; Taiwan; veterinary medicine; Animals; Chickens; Cricetinae; Diet, High-Fat; Fatty Liver; Insulin Resistance; Lipid Metabolism; Liver; Rodent Diseases; Taiwanjournal article10.1016/j.psj.2021.01.031336477172-s2.0-85101595301WOS:000637971600002https://api.elsevier.com/content/abstract/scopus_id/85101595301