國立臺灣大學醫學院外科賴逸儒2006-07-262018-07-112006-07-262018-07-112004-07-31http://ntur.lib.ntu.edu.tw//handle/246246/24531器官先經歷過短時間的缺血重灌流訓練後，再承受較長時間的缺血傷害時，器官耐 受力大為增加。這個現象稱為缺血前置訓練( ischemic Preconditioning)。已知 在模擬高山缺氧環境下飼養的動物, 其心肌對缺血的耐受性較好。過去對於缺 氧所誘發的前置訓練( Hypoxic Preconditioning) 的機制研究較少，本研究比較正 常氧壓飼養的大鼠和低氧飼養2週的大鼠，藉蛋白質體學方法,以二維電泳和質譜儀 分析其肝臟組織 探討前置缺氧訓練的機制。二維電泳和質譜儀分析的結果顯示低氧 飼養2週的大鼠肝臟中，大量表現myosin 及 actin等和胞器骨架或訊息傳遞有關的 蛋白質。吾人也以西方墨漬法分析確認myosin heavy chain (206DKa)的過度表現。 至於myosin heavy chain的表現是否具有功能上的意義，則需進一步探討。Preconditioning is defined as a phenomenon in which an organ or tissue is rendered resistant to a more sustained insult by prior exposure to brief periods of injury. Altitude training, used by endurance athletes to improve sea-level performance, is an exercise under exposure of chronic hypoxia. Exposure of the rat to a period of hypoxia increases the cardiac tolerance to subsequent ischemic insults. The mechanism of hypoxic preconditioning remains to be elucidated. We use the proteomics approach to study thoroughly the important factors involved in the mechanism of hypoxic preconditioning, Wistar rats exposed to 2 weeks of hypoxia were used, the liver tissue of experiment animals are harvested as target organ analysis. Two-dimensional gel electrophoresis was used to pick out the differentially-displayed protein spots for further analysis by mass spectrometry, and Protein database searching was performed. The proteins identified included myosin heavy chain, myosin polypeptide 9 (non-muscle type), myosin polypeptide 3, actin alpha1 and cytoplasmic beta-actin Western blot analysis confirmed the increased expression of myosin heavy chain (gi|8250661 ) in liver tissue of rats preconditioned by hypoxia.application/pdf79070 bytesapplication/pdfzh-TW國立臺灣大學醫學院外科HypoxiaLivermyosinreporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/24531/1/922314B002272.pdf