陳炳煇臺灣大學：機械工程學研究所錢睿宏Chien, Jui-HungJui-HungChien2007-11-282018-06-282007-11-282018-06-282007http://ntur.lib.ntu.edu.tw//handle/246246/60973本論文主要提出一新式的生物感測器,此生物檢測系統的主要原理是利用射頻共面波導的電路，並且在電路結構中植入奈米材料的共軛高分子，當病原體的核酸序列進入該共軛結構之後，會連接奈米金顆粒，進而改變射頻共面波導的電磁行為，達到檢測的目的。實驗結果顯示該感測器在低濃度的DNA或是蛋白質的檢測中均能達到良好的結果。This study presents a novel detection method for protein and DNA with multi-layer AuNPs to enhance overall detection sensitivity. Since the electromagnetic behaviors of the sensors can be altered by utilizing the existence of multilayer AuNPs in the biosensor, the change of the bandwidth of the sensing circuits can be the critical factor in evaluating the performance of the proposed biosensor. The measured 3-dB bandwidth of the original biosensor is about 18.8 GHz, which is close to the simulated results. The change in bandwidth for multi-layer AuNPs, which is formed by complementary target DNA, exceeds that of the double-layer AuNPs by 0.5 GHz. The changes of the 3-dB bandwidth are proportional to the logarithmic values of the analyte concentrations. Furthermore, the detection limit of the developed biosensor for the DNA set in this essay is 10pM, while it is 1ng/μL for the protein in this essay. In addition, with the temperature controlling system, single mutation of target DNA could be distinguished as the tested target DNA with an approximated length of 27 bps. The experimental results in this study indicate that this biosensor and chemical detection methodology are successful. As health care becomes much more essential in modern life, this biosensor has potential applications in a screening kit for recognizing, sensing, and quantifying biomolecules in real samples.Table of Contents Acknowledgement I Abstract II Nomenclature IV Table of Content VI List of Tables IX List of Figures X Chapter 1 Introduction 1 1.1 General Remarks 1 1.2 Motivation and Objectives 3 1.3 Literature Survey 6 1.3.1 Labeled Gold Nanoparticle on Antibody/DNA as the Bioreceptor 6 1.3.2 Low-Pass Filter as the Transducer 12 1.4 Contributions 12 1.4.1 The Improvement for Bioreceptor 12 1.4.2 The Novel Design of the Radio Frequency Transducer 13 1.5 Thesis Outline 14 Chapter 2 Design of the Coplanar Waveguide Filter 19 2.1 Principle of Coplanar Waveguide Circuits 19 2.1.1 Coplanar Waveguide Circuits 19 2.1.2 The Classification of Coplanar Waveguide 20 2.2 Principles of Filters 21 2.2.1 Passive Filters 21 2.2.2 Scattering Parameters 21 2.2.3 Definition of 3-dB Bandwidth 23 2.2.4 Dimensions of the Periodic CPW Circuits for Filters 23 2.3 Electromagnetic Simulation for the Proposed Biosensor 24 2.3.1 Simulation Tool 24 2.3.2 Simulation Procedures and Parameters for Biosensor 25 2.4 Materials and Fabrication of the Biosensor 26 2.4.1 Materials 26 2.4.2 Instruments for Biosensor Fabrication 27 2.4.3 Fabrication Process 27 Chapter 3 Protein Detection 41 3.1 Introduction to Immunoassay 41 3.1.1 Structure of Immunoglobulin G (IgG) 41 3.1.2 Principles of Immunoassay 42 3.1.3 Principles of Enzyme ImmunoAssay 43 3.2 Principles of Electrical Protein Detection Method 45 3.3 Experimental Procedures 46 3.3.1 Chemicals and Reagents 46 3.3.2 Solutions Preparation 47 3.3.3 Preparation of Gold Nanoparticles (AuNPs) 48 3.3.4 Preparation of Antibody Labeled on AuNPs 49 3.3.5 Immobilization of Monolayer AuNP on the Biosensor 49 3.3.6 Immobilization of Antibody on Monolayer AuNPs 49 3.3.7 Sandwich Immunoassay 50 3.3.8 Amplification of AuNPs on Antibody 50 Chapter 4 DNA Detection 61 4.1 Introduction to DNA 62 4.1.1 DNA Structure 62 4.1.2 Principles for Hybridization 63 4.1.3 Principles of Single Base-pair Mismatch Detection 63 4.2 Principles of Electrical Detection for DNA Hybridization 65 4.3 Experimental Procedures 66 4.3.1 Chemicals and Reagents 66 4.3.2 Solutions Preparation 67 4.3.3 Preparation of Gold Nanoparticles (AuNPs) 67 4.3.4 Establishment of Monolayer AuNPs 68 4.3.5 Establishment of Double-layer AuNPs 68 4.3.6 Establishment of Triple-layer AuNPs 69 Chapter 5 Results and Discussion 82 5.1 Simulated Results of the Biosensor 82 5.2 On-wafer Measured Results of the Biosensor 83 5.3 Protein Detection 84 5.4 DNA Detection 85 Chapter 6 Conclusions and Future Prospects 100 References 102 Appendix 1125556225 bytesapplication/pdfen-US射頻奈米金RFAuNPsthesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/60973/1/ntu-96-F92522104-1.pdf