Hsu P.-WChen C.-LTseng H.-WWo A.M.ANDREW WO2021-08-052021-08-052018https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079780161&partnerID=40&md5=29ea3517bfb82bd8bde8338baa02ac0dhttps://scholars.lib.ntu.edu.tw/handle/123456789/577048The emergence of next-generation sequencing (NGS) has propelled genomic analysis into a new era. However, the bottleneck is the library preparation which, for most cases, is labor-intensive and operator-dependent. In this study, a centrifugal-based microfluidic design enables on-chip fragmentation, end repair, A-addition, and ligation. Key features include multiplex processing, substantial reduction in reagent usage, low (?250 pg) input DNA quantity and reliable fluidic control. With simple to operate and low-cost microfluidic chip, the design demonstrated the potential for automated handling for multiplex library preparation for various applications. Copyright ? (2018) by Chemical and Biological Microsystems Society. All rights reserved.Centrifugation; Fluidic devices; Automated handling; Centrifugal microfluidics; Genomic analysis; Low sample input; Microfluidic chip; Next-generation sequencing; Sample preparation; Substantial reduction; Microfluidicsconference paper2-s2.0-85079780161