Lin HLin T.-YLin J.-AKUAN-CHEN CHENGSantoso S.PChou C.-HHsieh C.-W.2022-04-252022-04-25202120763921https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116759475&doi=10.3390%2fantiox10101589&partnerID=40&md5=b5696675f55b1b2964003732044093bchttps://scholars.lib.ntu.edu.tw/handle/123456789/606172Advanced glycation end products (AGEs) can induce oxidative stress and inflammation. AGEs are major risk factors for the development of many aging-related diseases, such as cancer and diabetes. In this study, Pholiota nameko polysaccharides (PNPs) were prepared from water extract of P. nameko via graded alcohol precipitation (40%, 60%, and 80% v/v). We explored the in vitro antiglycation ability of the PNPs and inhibition of methylglyoxal (MG)-induced Hs68 cell damage. In a bovine serum albumin (BSA) glycation system, PNPs significantly inhibited the formation of Amadori products. Fluorescence spectrophotometry revealed that the PNPs trapped MG and reduced MG-induced changes in functional groups (carbonyl and ε-NH2) in the BSA. Pretreating Hs68 cells with PNPs enhanced the cell survival rate and protected against MG-induced cell damage. This was due to decreased intracellular ROS content. PNPs thus mitigate skin cell damage and oxidative stress resulting from glycation stress, making them a potential raw material for antiaging-related skincare products. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Advanced glycation end productsAntiglycationCell agingGlycation stressHuman dermal fibroblastsPholiota namekoPolysaccharide[SDGs]SDG3[SDGs]SDG6journal article10.3390/antiox101015892-s2.0-85116759475