Tseng T.-Y.Chen W.-W.Chu I.-T.Wang C.-L.Chang C.-C.MEI-CHUN LINPEI-JEN LOUChang T.-C.2020-02-132020-02-1320182045-2322https://scholars.lib.ntu.edu.tw/handle/123456789/458529Using time-gated fluorescence lifetime imaging microscopy, significantly more signals from 3,6-bis(1-methyl-2-vinyl-pyridinium) carbazole diiodide (o-BMVC) foci, characterized by the longer fluorescent decay time of o-BMVC, were detected in six types of cancer cells than in three types of normal cells. Accumulating evidence suggested that the o-BMVC foci are mainly the G-quadruplex foci. The large contrast in the number of o-BMVC foci can be considered as a common signature to distinguish cancer cells from normal cells. Further study of tissue biopsy showed that the o-BMVC test provides a high accuracy for clinical detection of head and neck cancers. ? 2018, The Author(s).[SDGs]SDG33,6-bis(1-methyl-4-vinylpyridium)carbazole diiodide; carbazole derivative; fluorescent dye; guanine quadruplex; pyridinium derivative; case control study; chemistry; fluorescence microscopy; genetic procedures; genetics; head and neck tumor; human; metabolism; mouth; pathology; procedures; tumor cell culture; Biosensing Techniques; Carbazoles; Case-Control Studies; Fluorescent Dyes; G-Quadruplexes; Head and Neck Neoplasms; Humans; Microscopy, Fluorescence; Mouth; Pyridinium Compounds; Tumor Cells, Culturedjournal article10.1038/s41598-018-34378-8303821302-s2.0-85055780831