HSUAN-CHEN WU2018-09-102018-09-102011http://www.scopus.com/inward/record.url?eid=2-s2.0-84883807783&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/362217https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883807783&partnerID=40&md5=58638cb55eed8e2f534444a1033ee7d4This paper reports a unique approach to immobilize mammalian cell populations (mouse myeloma NS0) through in situ gelation of calcium alginate triggered by an electrical signal under physiologically relevant conditions. This is the first experimental observation to evaluate the electrically triggered assembly of calcium alginate gel for entrapping and culturing mammalian cells. Subsequent cell viabilities immediately after electrodeposition and after three days of culturing are studied. Our cell assembly strategy is applicable to fragile mammalian cells and can be used for in vitro study of dynamic cellular processes and cell-based assays under a microfluidic environment. Copyright © (2011) by the Chemical and Biological Microsystems Society.Calcium alginate; Cell culture; Electrodeposition; Mammalian cell; MicrofluidicsCalcium alginate; Calcium alginate gels; Cell-based assays; Cellular process; Electrical signal; Mammalian cells; Micro-fluidic devices; Microfluidic environment; Biocompatibility; Cell culture; Cell proliferation; Electrodeposition; Gelation; Microfluidics; Calciumconference paper2-s2.0-84883807783