Chiang, Yuan-MingYuan-MingChiangLiu, Hong-JeHong-JeLiuLin, Sin-RueiSin-RueiLinHuang, Jiun-WoeiJiun-WoeiHuangLee, Shu-ShengShu-ShengLeeHsiang-Chieh LeeCHIH-KUNG LEE2025-09-222025-09-222025https://www.scopus.com/record/display.uri?eid=2-s2.0-105014505074&origin=resultslisthttps://scholars.lib.ntu.edu.tw/handle/123456789/732224Adaptive optics technology was originally developed for astronomical observations to correct atmospheric disturbances and improve imaging quality. Recently, it has been widely adopted in fields such as optical communications and microscopy. Our research group has developed a DMD-assisted lateral shearing interferometer for wavefront sensing and correction. This system combined a digital micromirror device (DMD) and a lateral shearing interferometer operated by a shear plate and using a five-step phase-shifting method. In this study, we used a DMD with high resolution imaging (1080×1920) and a high refresh rate, to make the phase-shifting and the optical aberration correction more efficient. In addition, we integrated a designed K-mirror into the part of the wavefront sensing to utilize its ability to rotate images to simplify the optical path of the interferometer. This approach enhanced the stability of the system, and enabled wavefront gradient measurements in two orthogonal directions. Hence, a 2-D wavefront was accurately reconstructed and measured. The wavefront compensation was achieved by using computer generated holography (CGH), which allowed independent control of wavefront amplitude, phase and spatial frequency of the hologram. In the optical microscopy field, this system can compensate for the aberrations encountered during light propagation through inhomogeneity of material, which can lead to optical distortions, and enhance image quality.adaptive optics (AO)AO-microscopydigital micromirror device (DMD)Fourier opticsholographyLee hologram methodmicroscopyphase-shifting methodspatial light modulator (SLM)two-axis shearing interferometerwavefront aberrationswavefront correctionwavefront sensingAn adaptive optics microscopy system based on a DMD-assisted lateral shearing interferometerconference paper10.1117/12.3050793