2017-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/662746摘要：有鑒於在進口栽培介質及植株附帶栽培介質中檢出之重要植物檢疫線蟲有比例逐漸增加之趨勢，且為求有效預防高風險性外來種之入侵與散佈，本計劃擬針對國際間既存之快速分子檢測技術進行完整評估，同時開發符合我國檢疫及防疫需求之實用檢測流程與方法。本計畫將開發葉芽線蟲屬內種間之PCR快速分子檢測技術，建立莖線蟲屬內重要檢疫線蟲之分子檢驗作業流程，並開發用於鑑定檢疫線蟲之DNA生物晶片，以供未來檢疫人員快速鑑定相關檢疫線蟲使用。<br> Abstract: Due to the increasing detection of important plant parasitic nematodes in the inspected importing planting materials and in the planting materials attached to the importing plants, the importance of applying effective and efficient inspection and identification methods at quarantine have been elevated. To prevent the entrance of high risk quarantine nematode pests, the goal of this project is to evaluate existing detections assays and develop suitable protocol for the detection of the important nematodes i n Ditylenchus and Aphelenchoides genus. This project will evaluate all the existing DNA based molecular detection assays targeting the quarantine nematodes, develop effective and efficient molecular assays and optimize the best available assay to meet our quarantine inspection needs. Also, this project will develop a DNA biochip, providing quarantine inspection tool for rapid identification of the important quarantine nematodes.線蟲分子檢測生物晶片nematodemolecular detectionbiochip