Yang J.-TChen Y.-JHuang C.-WWang Y.-CMersmann H.JWang P.-HPEI-HWA WANGSHIH-TORNG DING2022-04-252022-04-25202120726643https://www.scopus.com/inward/record.uri?eid=2-s2.0-85113834604&doi=10.3390%2fnu13072315&partnerID=40&md5=24f8613ed84457b2f864992a17200acchttps://scholars.lib.ntu.edu.tw/handle/123456789/605862Tetranectin (TN), a plasminogen-binding protein originally involved in fibrinolysis and bone formation, was later identified as a secreted adipokine from human and rat adipocytes and positively correlated with adipogenesis and lipid metabolism in adipocytes. To elucidate the nutritional regulation of adipogenic TN from diets containing different sources of fatty acids (saturated, n-6, n-3) in adipocytes, we cloned the coding region of porcine TN from a cDNA library and analyzed tissue expressions in weaned piglets fed with 2% soybean oil (SB, enriched in n-6 fatty acids), docosahexaenoic acid oil (DHA, an n-3 fatty acid) or beef tallow (BT, enriched in saturated and n-9 fatty acids) for 30 d. Compared with tissues in the BT-or SB-fed group, expression of TN was reduced in the adipose, liver and lung tissues from the DHA-fed group, accompanied with lowered plasma levels of triglycerides and cholesterols. This in vivo reduction was also confirmed in porcine primary differentiated adipocytes supplemented with DHA in vitro. Then, promoter analysis was performed. A 1956-bp putative porcine TN promoter was cloned and transcription binding sites for sterol regulatory-element binding protein (SREBP)-1c or forkhead box O proteins (FoxO) were predicted on the TN promoter. Mutating binding sites on porcine TN promoters showed that transcriptional suppression of TN by DHA on promoter activity was dependent on specific response elements for SREBP-1c or FoxO. The inhibited luciferase promoter activity by DHA on the TN promoter coincides with reduced gene expression of TN, SREBP-1c, and FoxO1 in human embryonic kidney HEK293T cells supplemented with DHA. To conclude, our current study demonstrated that the adipogenic TN was negatively regulated by nutritional modulation of DHA both in pigs in vivo and in humans/pigs in vitro. The transcriptional suppression by DHA on TN expression was partly through SREBP-1c or FoxO. Therefore, down-regulation of adipogenic tetranectin associated with fibrinolysis and adipogenesis may contribute to the beneficial effects of DHA on ameliorating obesity-induced metabolic syndromes such as atherosclerosis and adipose dysfunctions. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.Adipose tissuesDocosahexaenoic acid (DHA)Forkhead box O protein (FoxO)PigsSterol regulatory element-binding protein-1c (SREBP-1c)Tetranectincholesteroldocosahexaenoic acidsterol regulatory element binding proteinsterol regulatory element binding protein 1ctetranectintranscription factor FOXOtriacylglycerolforkhead transcription factorlectinadipocyteadipogenesisanimal cellanimal experimentanimal tissueArticlebinding sitecholesterol blood levelcontrolled studyDNA libraryembryogene expressionhumanhuman cellin vitro studymalenonhumanpigpromoter regionprotein expressiontriacylglycerol blood levelanimaldrug effectfibrinolysisgeneticsHEK293 cell linemetabolismnutritionAdipocytesAdipogenesisAnimalsDocosahexaenoic AcidsFibrinolysisForkhead Transcription FactorsHEK293 CellsHumansLectins, C-TypeNutritional Physiological PhenomenaSterol Regulatory Element Binding ProteinsSwine[SDGs]SDG3journal article10.3390/nu13072315343718222-s2.0-85113834604