2023-01-012024-05-16https://scholars.lib.ntu.edu.tw/handle/123456789/670025辨認和解開 hiPSCs分化大腦類器官之連接組的結構和功能方面的奧秘，將在當前的研究和臨床實踐中帶來全新且具革命性的轉變。為了實現了解 hiPSCs 分化大腦類器官的神經元同步和興奮性的目標，本研究計劃的目的是構建和優化定制的雙光子顯微鏡，以解構大腦類器官的結構並構建具有單細胞解析度之大腦類器官中神經元活動的功能性分析系統。為此，通過開發此雙光子顯微平台，該研究計劃的科學目標是： 1. 了解 hiPSCs 衍生的大腦類器官中的神經元同步性和興奮性，以及類器官模型之間如何形成耦合。 2. 了解自閉症譜系障礙 (ASD) 的起源以及它如何影響附近的神經系統。 Identifying and unraveling the mystery of the structural and functional aspects of hiPSCs-derived brain organoids’ connectome will usher a new paradigm shift in the present research and clinical practice. In order to achieve the goal of understand neruonal synchronization and excitability in hiPSCs-derived brain organoids, the objective of this research proposal is to build and optimize a customized two photon microscope so as to comprehend the structural readout of brain organoids and to construct a function readout system of neuronal activity in the brain organoids with single-cell resolution. To this end, by developing the two photon microscopy platforms, the scientific aims of the research program are: 1. To understand neuronal synchronization and excitability in hiPSCs-derived cerebral organoids and how coupling is formed between in the organoid-model. 2. To comprehand how Autism spectrum disorder (ASD) originates and how it affects the nearby nervous system.建立及優化偵測活體大腦神經元的同步活性及興奮度;Establish and optimize imaging tools for detecting neuronal synchronization and excitability in vivo