Wu, Chih YuChih YuWuYU-CHIH CHIANGChristy, JaneJaneChristyABEL PO-HAO HUANGChang, Nai YunNai YunChangWenny, Chiu Y.-C.Chiu Y.-C.WennyChiu, Yu ChihYu ChihChiuYang, Yen ChingYen ChingYangChen, Po ChunPo ChunChenWang, Peng YuanPeng YuanWangHSIEN-YEH CHEN2021-08-302021-08-302021-05-012079-6412https://scholars.lib.ntu.edu.tw/handle/123456789/579853Modifications of biomaterials based on the combination of physical, chemical, and bio-logical cues for manipulating stem cell growth are needed for modern regenerative medicine. The exploitation of these sophisticated modifications remains a challenge, including substrate limitation, biocompatibility, and versatile and general cues for stem cell activities. In this report, a vapor-phase coating technique based on the functionalization of poly-p-xylylene (PPX) was used to generate a surface modification for use with stem cells in culture. The coating provided the ability for covalent conjugation that immobilized bone morphogenetic protein 2 (BMP-2) and fibroblast growth factor 2 (FGF-2), and the modified coating surfaces enabled direct stem cell differentiation and controlled proliferation because of the specific activities. The ligations were realized between the growth factors and the maleimide-modified surface, and the conjugation reactions proceeded with high specificity and rapid kinetics under mild conditions. The conjugation densities were approximately 140 ng·cm−2 for BMP-2 and 155 ng·cm−2 for FGF-2. Guiding the activities of the human adipose-derived stem cells (hADSCs) was achieved by modifying surfaces to promote the hADSC differentiation capacity and proliferation rate. The reported coating system demonstrated biocompatibility, substrate-independent conformity, and stability, and it could provide an effective and versatile interface platform for further use in biomedical applications.Biointerface | CVD polymerization | Growth factor | Stem cells | Surface modificationBiointerface; CVD polymerization; Growth factor; Stem cells; Surface modification[SDGs]SDG3journal article10.3390/coatings110505822-s2.0-85106999094https://scholars.lib.ntu.edu.tw/handle/123456789/570004