YU-TING CHANGMING-SHIANG WUCHIA-TUNG SHUNMING-TSAN LINMING-CHU CHANGLin J.-T.2021-02-042021-02-0420020172-6390https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036375148&partnerID=40&md5=4246cbc6ade287a173d7ff1b200a8e13https://scholars.lib.ntu.edu.tw/handle/123456789/548412Background/Aims: Interleukin-1β (IL-1β) plays an important role in gastric inflammation and physiology. Functional polymorphisms of IL-1β gene have been related to different risks of gastric cancer and duodenal ulcer but their role in gastric ulcer remains unknown. In this study, we investigate a plausible association between gastric ulcer and the polymorphisms in the IL-1β and IL-1 receptor antagonist (IL-1RN) genes. The relationships among the cytokine genotyping, other environmental risks such as Helicobacter pylori infection and smoking, and clinical characteristics of gastric ulcer were also determined. Methodology: Peripheral blood DNAs from 120 unrelated Taiwan Chinese patients with gastric ulcer and 238 ethnically matched healthy controls were genotyped for the promoter (position -31 and -511) and Taq I polymorphism (position +3954) in the IL-1β gene and the variable number of tandem repeats polymorphisms in intron 2 of the IL-1RN gene. The status of Helicobacter pylori infection was determined by serology. Results: The seropositive rate of Helicobacter pylori (95/120 vs. 134/238, OR: 2.95, 95%CI 1.77-4.91), habitual smoking (67/120 vs. 82/238, OR: 2.40, 95%CI 1.54-3.77) and blood group O (63/120 vs. 98/238, OR: 1.55, 95%CI 1.0-2.41) were significantly higher in patients with gastric ulcer than controls. The distributions of allele frequencies of IL-1β (-31 C/T or -511 C/T or +3954) and IL-1RN were similar between patients with gastric ulcer and controls. No significant differences were observed, including those analyzed after stratification of the infected population and by the ulcer subgroup. Conclusions: Our data suggested that Helicobacter pylori infection, cigarette smoking, and blood group O are risk factors of gastric ulcer and IL-1β or IL-1RN polymorphisms do not influence susceptibility to gastric ulcer in a Taiwanese population.[SDGs]SDG3cytokine; DNA; interleukin 1 receptor blocking agent; interleukin 1beta; adult; article; Chinese; cigarette smoking; clinical feature; controlled study; DNA polymorphism; environmental factor; ethnic group; female; genotype; habit; Helicobacter infection; Helicobacter pylori; human; intron; major clinical study; male; priority journal; promoter region; risk assessment; serology; stomach ulcer; Taiwan; variable number of tandem repeat; Aged; Female; Genetic Predisposition to Disease; Helicobacter Infections; Helicobacter pylori; Humans; Interleukin-1; Male; Middle Aged; Polymorphism, Genetic; Risk Factors; Stomach Ulcerjournal article122399702-s2.0-0036375148