Teo, Kai-FaKai-FaTeoChen, Der-YuanDer-YuanChenHsu, Jeh-TingJeh-TingHsuLai, Yi-HuaYi-HuaLaiChang, Ching-KunChing-KunChangPO-REN HSUEHLan, Joung-LiangJoung-LiangLanHsu, Jye-LinJye-LinHsu2023-02-142023-02-142023-011752-8054https://scholars.lib.ntu.edu.tw/handle/123456789/628084An efficient host immune response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) appears to be crucial for controlling and resolving this viral infection. However, many studies have reported autoimmune characteristics in severe COVID-19 patients. Moreover, clinical observations have revealed that COVID-19-associated acute distress respiratory syndrome shares many features in common with inflammatory myopathy including interstitial lung disease (ILD), most particularly rapidly progressive (RP)-ILD. This study explored this phenomenon by seeking to identify and characterize myositis-specific and related autoantibodies in 25 COVID-19 patients with mild or severe symptoms. Line blot analysis with the EUROLINE Myopathies Ag kit identified 9 (36%) patients with COVID-19 with one or more autoantibodies against several myositis-related antigens (Jo-1, Ku, Mi-2β, PL-7, PL-12, PM-Scl 75, PM-Scl 100, Ro-52, and SRP); no anti-MDA5 antibodies were detected. As the presence of antibodies identified by line blots was unrelated to disease severity, we further characterized the autoantibodies by radioimmunoassay, in which [35 S]methionine-labeled K562 cellular antigens were precipitated and visualized by gel electrophoresis. This result was confirmed by an immunoprecipitation assay and immunoblotting; 2 patients exhibited anti-Ku70 and anti-Ku80 antibodies. Our data suggest that it is necessary to use more than one method to characterize and evaluate autoantibodies in people recovered from COVID-19, in order to avoid misinterpreting those autoantibodies as diagnostic markers for autoimmune diseases.enGENE 5; JAPANESE PATIENTS; ANTIBODIES; ASSOCIATION; DERMATOMYOSITIS[SDGs]SDG3journal article10.1111/cts.13434362716472-s2.0-85141430698WOS:000876120300001https://api.elsevier.com/content/abstract/scopus_id/85141430698