2011-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/644698摘要：根據九八年國健局公佈的統計資料，大腸直腸癌已經成為台灣地區癌症新增加個案數的首位（據估計每年新增加的個案數約10248 位）。儘管現在醫療的進步，這群患者的平均五年存活率也只有55%，也就是說約有一半的病人會因腫瘤復發而死亡。而復發最常見的位置就是肝、肺、骨頭等遠端轉移。因此，了解會造成遠端轉移的可能基因及其相關的分子機轉，對癌症病患的治療就顯得相當的重要。我們之前的研究，皆只針對單一基因或蛋白色質（例如TWEAK, CTGF,Cyr61）在癌症的角色扮演，加以功能性與機轉性的探討，這部份已取得相當的操作經驗與成果發表。然而我們也很清楚癌症的轉移過程，是需要不同的基因彼此相互作用，才能讓癌細胞在其它地方生長達成轉移的現象。因此，我們初步利用Microarray 的方法，來比較大腸直腸癌母細胞株（KM12C, SW620）和肝轉移細胞株（KM12L4A, SW620L1）彼此在基因表現上的差異、找出跟癌症轉移相關的基因；同時設計一系列的實驗，來驗證他們可能的分子機轉及其在臨床病人上預測復發與否的署名基因之應用。<br> Abstract: According to the statistical data from Taiwan Cancer Registry 2009, colorectalcancer has be ranked No. 1 for newly increased cases per year, approximately 10248new cases being diagnosed annually. Regardless of the progression of modernmedicine, the average 5-year survival rate was still around 55%. It means that half ofpatients with diagnosis of colorectal cancer will die of recurrence. The most commonsites of recurrence were distal metastases to liver, lung or bone. Therefore, it isimportant for patients’treatment to understand which gene results in cancermetastasis and how the underlying molecular mechanism decides its function.Our prior projects were one by one to study the role of specific single gene incancer, including connective tissue growth factor (CTGF), cysteine-rich 61(Cyr61)and tumor necrosis factor-like weak inducer of apoptosis (TWEAK) and throughthese works, we had accumulated lots of experiences in research design and paperpublication. However, we all know that the process of metastasis needs mutual actionand cooperation of different genes and it could not be decided by the accomplishmentof single gene. Hence, the initial concept of this study is to comparing the differenceof gene expression in parent cell lines (KM12C, SW620) and their liver-metastasiscell lines (KM12L4A, SW620L1) by utilizing microarray. Later, the furtherexperiments would be needed to study and confirm our draft proposal about the possiblerole of metastasis-associated genes in colorectal cancer and translate them into genesignature for predicting patients’recurrence in clinical situations.