Chen, Bo-ChingBo-ChingChenLiao, Chung-MinChung-MinLiao2010-01-062018-06-292010-01-062018-06-292004http://ntur.lib.ntu.edu.tw//handle/246246/175996The present study couples the Michaelis-Menten (M-M) type flux and the Fick's type of dynamic mass transfer flux to arrive at the Best equation to quantitatively model the transport and biouptake mechanism of the gills of freshwater tilapia (Oreochromis mossambicus) exposed to waterborne arsenic (As). We conducted a 15-day uptake/depuration bioassay to examine the accumulation kinetics of As in tilapia gills by incorporating a bioconcentration model to obtain the steady-state and dynamic bioconcentration factors. A diffusion-based permeability can be calculated using the physiological and allometric-related parameters. The bioaffinity parameter and the limiting uptake flux in M-M equation are acquired by fitting the experimental values from published literature. The biouptake rate incorporating with bioavailability number is examined to better understand the effects of variabilities of field circumstances on biouptake flux. A linear relationship between As biouptake rate and As concentration in ambient water is obtained. The fitted bioaffinity parameter and limiting uptake flux were 3.07 mg l -1 and 2.17 mg l -1 day -1, respectively, suggesting a low As binding affinity of tilapia gills yet a relative high binding capacity was obtained. The As permeability through tilapia gills membrane decreased from 1.42 μm day -1 to a steady-state value of 0.82 μm day -1 after 2 months, indicating the nonequilibrium aspects of biouptake processes involved. © 2004 Elsevier B.V. All rights reserved.application/pdf386933 bytesapplication/pdfen-USArsenic; Bioavailability; Biouptake; Tilapia[SDGs]SDG6[SDGs]SDG15aquaculture; arsenic; biological uptake; Oreochromis; Oreochromis mossambicus; Tilapiajournal article10.1016/j.aquaculture.2004.09.0092-s2.0-9744234493http://ntur.lib.ntu.edu.tw/bitstream/246246/175996/1/45.pdf