2014-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/684287摘要：嘉德麗雅蘭原生於中南美洲，其花型大且花色變化多，但其對乙烯耐受性極低，切花瓶插壽命約為七天。為了克服嘉德麗雅蘭切花花期過短之問題，本計畫期望利用生物技術，調控嘉德麗雅蘭對乙烯之敏感度。本計畫首先將建立嘉德麗雅蘭完整的基因轉殖及再生系統，接著將乙烯訊息傳導路徑負向調控因子 CTR1 (constitutive triple response 1) 基因過量表現於嘉德麗雅蘭，以阻撓乙烯訊息傳導路徑，培育出具有較長花期之新品種。本年度的工作項目包括建立嘉德麗雅蘭再生及基因轉殖系統，並進行延長嘉德麗雅蘭花期轉殖質體之構築。預期所建立之嘉德麗雅蘭基因轉殖系統，可作為改良嘉德麗雅蘭性狀之基因轉殖平台；本計畫所構築之 CTR1 過量表現載體，亦可應用於其他作物延長保鮮期之基因轉殖。<br> Abstract: Cattleya orchids are originated from Costa Rica to tropical South America. Flowers of Cattleya are very attractive because their large size and variable colors. Unfortunately, flowers of Cattleya are very sensitive to ethylene. To prolong the vase life of cut Cattleya flowers, manipulation of signal transduction in Cattleya flowers for reduction of ethylene sensitivity will be conducted in this project via genetic transformation. Studies on the regeneration and Agrobacterium-mediated transformation systems will be set up this year. The cDNA of negative regulator CTR1 driven by CaMV 35S promoter will be constructed into a binary vector for Agrobacterium-mediated transformation of Cattleya. The transformation platform established in this project would be applicable to transformation of genes related to other phenotypes. Moreover, the plasmid constructed in this project may be transformed into other crops to prolong their shelf-lives.嘉德麗雅蘭花瓣老化乙烯訊息傳導Cattleyapetal senescenceethylene signal transduction