Wei, L.-G.L.-G.WeiChang, H.-I.H.-I.ChangWang, Y.Y.WangHsu, S.-H.S.-H.HsuDai, L.-G.L.-G.DaiFu, K.-Y.K.-Y.FuDai, N.-T.N.-T.DaiSHAN-HUI HSU2020-03-022020-03-022019https://scholars.lib.ntu.edu.tw/handle/123456789/467551Background. A tissue-engineered skin substitute, based on gelatin (‘‘G’’), collagen (‘‘C’’), and poly(ε-caprolactone) (PCL; ‘‘P’’), was developed. Method. G/C/P biocomposites were fabricated by impregnation of lyophilized gelatin/collagen (GC) mats with PCL solutions, followed by solvent evaporation. Two different GC:PCL ratios (1:8 and 1:20) were used. Results. Differential scanning calorimetry revealed that all G/C/P biocomposites had characteristic melting point of PCL at around 60 ?C. Scanning electron microscopy showed that all biocomposites had similar fibrous structures. Good cytocompatibility was present in all G/C/P biocomposites when incubated with primary human epidermal keratinocytes (PHEK), human dermal fibroblasts (PHDF) and human adipose-derived stem cells (ASCs) in vitro. All G/C/P biocomposites exhibited similar cell growth and mechanical characteristics in comparison with C/P biocomposites. G/C/P biocomposites with a lower collagen content showed better cell proliferation than those with a higher collagen content in vitro. Due to reasonable mechanical strength and biocompatibility in vitro, G/C/P with a lower content of collagen and a higher content of PCL (GCLPH) was selected for animal wound healing studies. According to our data, a significant promotion in wound healing and skin regeneration could be observed in GCLPH seeded with adipose-derived stem cells by Gomori’s trichrome staining. Conclusion. This study may provide an effective and low-cost wound dressings to assist skin regeneration for clinical use. ? 2019 Wei et al.Adipose-derived stem cells; Collagen; Gelatin; Poly(ε-caprolactone)(PCL); Skin tissue engineering[SDGs]SDG3collagen; cytoskeleton protein; gelatin; polycaprolactone; adipose derived stem cell; adult; animal experiment; animal model; Article; biocompatibility; BJ [Human fibroblast] cell line; cell culture; cell density; cell encapsulation; cell isolation; cell proliferation; child; composite graft; differential scanning calorimetry; extracellular matrix; female; foreskin cell line; high performance liquid chromatography; human; human cell; immunohistochemistry; keratinocyte; mouse; nonhuman; protein secretion; regenerative medicine; scanning electron microscopy; skin regeneration; tensile strength; tissue engineering; wound healingjournal article10.7717/peerj.63582-s2.0-85063616270https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063616270&doi=10.7717%2fpeerj.6358&partnerID=40&md5=9b9f7aa9b378d36ce7a95db39ef5d425