SHIH-JUNG CHENGChang, Chi-FengChi-FengChangHUI-HSIN KOJANG-JAER LEEHSIN-MING CHENWang, Huei-JenHuei-JenWangLin, Hsiao-ShanHsiao-ShanLinCHUN-PIN CHIANG2021-10-182021-10-1820181043-3074https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030319443&doi=10.1002%2fhed.24958&partnerID=40&md5=e488d6da3d47dab236d86980ab93508fhttps://scholars.lib.ntu.edu.tw/handle/123456789/584972Background: Effective biomarkers for oral cancer screening are important for early diagnosis and treatment of oral cancer. Methods: Oral epithelial cell samples collected by mouth rinse were obtained from 65 normal control subjects, 108 patients with oral potentially malignant disorders, and 94 patients with oral squamous cell carcinoma (OSCC). Methylation levels of zinc-finger protein 582 (ZNF582) and paired-box 1 (PAX1) genes were quantified by real-time methylation-specific polymerase chain reaction after bisulfite conversion. Results: An abrupt increase in methylated ZNF582 (ZNF582m) and PAX1 (PAX1m) levels and positive rates from mild dysplasia to moderate/severe dysplasia, indicating that both ZNF582m and PAX1m are effective biomarkers for differentiating moderate dysplasia or worse (MODY+) oral lesions. When ZNF582m/PAX1m tests were used for identifying MODY+ oral lesions, the sensitivity, specificity, and odds ratio (OR) were 0.65/0.64, 0.75/0.82, and 5.6/8.0, respectively. Conclusion: Hypermethylated ZNF582 and PAX1 genes in oral epithelial cells collected by mouth rinse are effective biomarkers for the detection of oral dysplasia and oral cancer. ? 2017 Wiley Periodicals, Inc.[SDGs]SDG3mouthwash; unclassified drug; zinc finger protein; zinc-finger protein 582; biological marker; kruppel like factor; paired box transcription factor; PAX1 transcription factor; tumor marker; ZNF582 protein, human; adult; Article; cancer diagnosis; cancer risk; comparative study; controlled study; DNA methylation; dysplasia; female; gene; gene sequence; genetic association; human; longitudinal study; major clinical study; male; mouth cancer; mouth lesion; mouth squamous cell carcinoma; oral dysplasia; paired box 1 gene; patient selection; priority journal; sensitivity and specificity; DNA methylation; genetics; leukoplakia; middle aged; mouth; mouth tumor; pathology; precancer; squamous cell carcinoma; Adult; Biomarkers; Biomarkers, Tumor; Carcinoma, Squamous Cell; DNA Methylation; Female; Humans; Kruppel-Like Transcription Factors; Leukoplakia, Oral; Male; Middle Aged; Mouth; Mouth Neoplasms; Paired Box Transcription Factors; Precancerous Conditionsjournal article10.1002/hed.24958289606392-s2.0-85030319443