SUNG-HSIN KUOWeng W.-H.Chen Z.-H.PING-NING HSUMING-SHIANG WUCHUNG-WU LINJeng H.-J.KUN-HUEI YEHTsai H.-J.Chen L.-T.ANN-LII CHENG2020-03-032020-03-0320111045-2257https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052682676&doi=10.1002%2fgcc.20910&partnerID=40&md5=a6892627b4bb5442832f3c8adcc01283https://scholars.lib.ntu.edu.tw/handle/123456789/468869Although t(11;18)(q21;q21), t(1;14)(p22;q32), and a few other genetic mutations are specific markers for the Helicobacter pylori (HP)-independent status of gastric mucosa-associated lymphoid tissue (MALT) lymphoma, the molecular mechanisms responsible for HP-independence of gastric MALT lymphoma without such translocations and mutations remain uncharacterized. In the present study, we describe the establishment and characterization of a novel MALT lymphoma cell line, MA-1, which was derived from a gastric MALT lymphoma which was negative for both t(11;18)(q21;q21) and t(1;14)(p22;q32); the patient had failed HP eradication therapy and chemotherapy. The cell morphology and the immunophenotype of this cell line were similar to that of the original gastric MALT lymphoma. Comparative genomic hybridization analysis showed no significant gene copy number changes. Spectral karyotyping displayed a near-diploid chromosome content (48 < 2n>XY), with at least 13 chromosome structural abnormalities. Furthermore, fluorescence in situ hybridization analyses disclosed the existence of three sub-clones, characterized by t(14;18)(q32;q21)/IGH-BCL2, t(14;18)(q32;q21)/IGH-MALT1, and the presence of both chromosomal translocations in the same cell, respectively; whereas amplification of the genes CRAD9, TRAF2, and BCL10 were not found. In conclusion, we have established the first human gastric MALT lymphoma cell line, which is characterized by unusual and complex chromosome translocations and will be useful to explore further the molecular mechanisms of HP-independence in gastric MALT lymphoma. ? 2011 Wiley-Liss, Inc.[SDGs]SDG3amoxicillin; chlorambucil; clarithromycin; cyclophosphamide; omeprazole; prednisolone; protein; protein bcl 10; protein crad9; rituximab; tumor necrosis factor receptor associated factor 2; unclassified drug; vincristine; adult; article; bone marrow biopsy; cancer combination chemotherapy; cancer patient; cancer regression; case report; cell structure; chromosome 14q; chromosome 18q; chromosome translocation 1; chromosome translocation 11; chromosome translocation 14; chromosome translocation 18; comparative genomic hybridization; diploidy; disease free survival; drug substitution; drug treatment failure; drug withdrawal; epigastric pain; eradication therapy; female; fluorescence in situ hybridization; follow up; gene amplification; gene dosage; Helicobacter infection; Helicobacter pylori; histopathology; human; human cell; human tissue; immunohistochemistry; immunophenotyping; karyotype 46,XY; karyotyping; lymphadenopathy; lymphoma cell line; monotherapy; mucosa associated lymphoid tissue lymphoma; multiple cycle treatment; nuclear magnetic resonance imaging; pancytopenia; priority journal; stomach lymphoma; structural chromosome aberration; tumor associated leukocyte; tumor recurrence; Waldeyer ring; Cell Line, Tumor; Chromosomes, Human, Pair 14; Chromosomes, Human, Pair 18; Comparative Genomic Hybridization; Flow Cytometry; Gene Dosage; Helicobacter Infections; Helicobacter pylori; Humans; Immunohistochemistry; Immunophenotyping; In Situ Hybridization, Fluorescence; Lymphoma, B-Cell, Marginal Zone; Lymphoma, Non-Hodgkin; Male; Microsatellite Repeats; Middle Aged; Spectral Karyotyping; Stomach Neoplasms; Translocation, Genetic; Helicobacter pylorijournal article10.1002/gcc.20910218377082-s2.0-80052682676