WO-JAN TSENGHuang, S.-W.S.-W.HuangFang, C.-H.C.-H.FangHsu, L.-T.L.-T.HsuChen, C.-Y.C.-Y.ChenShen, H.-H.H.-H.ShenJENNY ZWEI-CHIENG CHANGJUI-SHENG SUNFENG-HUEI LIN2020-02-072020-02-0720190213-3911https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057553019&doi=10.14670%2fHH-18-013&partnerID=40&md5=d01637e2353cf879ad56b3d125c7a61bhttps://scholars.lib.ntu.edu.tw/handle/123456789/455293Summary. Objective. With the goal to explore a new approach to treat the early degenerative lesions of hyaline cartilage, we implanted in a porcine OA model a collagen-based scaffold containing chondroprogenitor cells derived from human bone marrow mesenchymal stem cells (hBM-MSCs). Experimental design. Porcine knee joints were subjected to anterior cruciate ligament (ACL) transection to surgically induce OA. After 4 months, the time necessary for the development of cartilage surface damage, animals were treated either wi t h t r ephi nat i on bone pl ug wr apped wi t h t he chondroprogeni c hBM-MSCs-embedded col l agen scaffold or microfractures alone. Histological and histomorphometric evaluations were performed at 5 months after surgery. Results. All animals subjected to ACL t r ansect i on showed ost eoar t hr i t i c changes including mild lateral femoral condyle or moderate medial femoral condyle ulcerations. After 14 days’ chondrogenic induction, hBM-MSCs seeded onto the scaffold showed expression of chondroprogeni tor markers such as SOX9 and COMP. At 5 months after the implantation, significant differences in the quality of the regenerated tissue were found between the hBM-MSCs-embedded scaffold group and the control group. Newly generat ed t i ssue was onl y observed at t he si t e of implantation with the hBM-MSCs-embedded scaffolds. Furthermore, histological examination of the generated tissue revealed evidence of cartilage-like tissue with lacuna formation. In contrast, fibrous layers or fissures were formed on the surface of the control knee joint. Conclusions. This study shows that xenogenic hBM-MSC derived chondroprogenitor scaffolds can generate new cartilage tissue in porcine articular cartilage and have the potential as a useful treatment option for osteoarthritis. ? Histology and Histopathology. All rights reserved.[SDGs]SDG3collagen; animal; articular cartilage; chondrogenesis; disease model; human; mesenchymal stem cell transplantation; osteoarthritis; physiology; pig; procedures; tissue engineering; tissue scaffold; xenograft; Animals; Cartilage, Articular; Chondrogenesis; Collagen; Disease Models, Animal; Heterografts; Humans; Mesenchymal Stem Cell Transplantation; Osteoarthritis; Swine; Tissue Engineering; Tissue Scaffoldsjournal article10.14670/HH-18-013299053612-s2.0-85057553019