2018-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/651762摘要：叢枝菌根共生 (arbuscular mycorrhiza symbiosis) 是指植物根部與球囊菌門 (Glomeromycota) 真菌的內共生關係。雖然叢枝菌根對促進植物生長的好處已被廣 泛報導，對於建立和維持此共生關係所需的分子機制仍未被完全了解。舉例來說 ，植物中有一些基因只會表達在有真菌存在的根部細胞中。他們的表現對於共生關 係的建立或許扮演重要的角色。然而，這些基因的表達如何受到調控仍未知。目前 已知，會被共生誘導表現的植物轉錄因子 (transcription factor) 往往對於建立 共生關係扮演重要角色。為了更了解轉錄因子在共生關係的建立中扮演的角色，我 們預期達成四項具體目標：(一) 藉由水稻轉錄組 (transcriptomes) 的分析，找 到被共生誘導表現的植物轉錄因子。這些植物轉錄因子的表現可在番茄中進一步檢 測，以確定是否同時在單子葉和雙子葉植物中具有重要性。(二) 利用反向遺傳學 (reverse genetics approach) 方法研究轉錄因子的功能。(三) 利用 DAP-seq 技 術進一步找到這些轉錄因子的目標基因。(四) 釐清在豆科植物中的兩個已知調控 途徑是否也在水稻和番茄中存在，並進一步研究這兩個途徑對於調控共生相關基因 表現的重要性。此研究結果可讓我們了解植物細胞如何藉由調控不同基因的表現以 建立共生關係。對於日後有效率利用真菌增進植物的營養吸收、產量和抗逆境的能 力則會有莫大的幫助。<br> Abstract: Arbuscular mycorrhiza (AM) symbiosis is the name given to the endosymbiotic association of a plant root and a fungus from the phylum Glomeromycota. More than 80% of vascular plants can be colonized by AM fungi. The benefit of mycorrhizal fungi for plant growth has been widely reported in different plant species, but the detailed molecular mechanism essential for establishing and maintaining symbiosis has not been clearly characterized. For example, a set of genes exclusively expressed in mycorrhizal roots and their expression might be essential for cellular reprogramming during AM symbiosis. However, the transcriptional regulation mechanism of these genes is not fully understood. Often, transcription factors with expression upregulated by AM symbiosis are important for AM symbiosis. To further understand the role of transcription factors in the establishment of AM symbiosis, this project aims to (1) identify AM-specific or induced transcription factors in rice by comparing the transcriptomes using RNA-seq technology (The expression of these candidate genes can be confirmed in tomato to determine any conservation between monocots and dicots.); (2) analyze the function of candidate transcription factors by reverse genetics; (3) identify the target genes regulated by the candidate transcription factors by DNA affinity purification sequencing (DAP-seq); and (4) clarify the contribution of two known pathways found in legumes for regulating AM-responsive gene expression in rice and tomato. The results of this study could help us understand how plant cells establish AM symbiosis by regulating the expression of different genes. This information will be helpful for future use of AM fungi to efficiently enhance nutrition absorption, yield and stress-tolerance of plants.叢枝菌根共生轉錄因子arbuscular mycorrhiza symbiosistranscription factor