TZYY-CHANG HODel Priore, Lucian V.Lucian V.Del PrioreHornbeck, RussellRussellHornbeck2023-03-212023-03-211997-07-0802713683https://scholars.lib.ntu.edu.tw/handle/123456789/629534Purpose. To determine the effect of mitomycin-C on confluent and non-confluent human retinal pigment epithelium (RPE) in tissue culture. Methods. The effect of mitomycin-C on confluent RPE was determined by treating first passage confluent cells with 0.01, 0.1, 1, 10, 100 or 1000 micromolar (μM) mitomycin-C for 1, 3, or 7 days. The cell viablility after treatment was determined by using an esterase stain. The effect of mitomycin-C on proliferating RPE was determined by incubating non-confluent cells with the above concentrations of mitomycin-C for 20 min, 1 hour or 24 hours. Results. Mitomycin-C can be toxic to a confluent RPE monolayer, and the LD50 is 421, 28.8 or 0.0632 μM when cells are continually exposed to mitomycin-C for 1, 3 or 7 days, respectively. Exposure to mitomycin-C at concentrations ≤ 10 μM for 20-60 min significantly inhibits proliferation of non-confluent RPE. A 24 hour exposure of RPE to 1 μM mitomycin-C markedly inhibits proliferation of non-confluent RPE with minimal toxicity to confluent RPE. Conclusions. Since exposure of human RPE to mitomycin-C for 24 hours can inhibit cell proliferation at concentrations which are well-tolerated by confluent RPE, mitomycin-C may be a suitable agent for inhibiting RPE proliferation in vivo.enCell proliferation | Human retinal pigment epithelium (HRPE) | Mitomycin-C | Proliferative vitreoretinopathy[SDGs]SDG6journal article10.1076/ceyr.16.6.572.507391921662-s2.0-0030908040https://api.elsevier.com/content/abstract/scopus_id/0030908040