Li H.-WMao J.-YLien C.-WWang C.-KLai J.-YMandal R.PHUAN-TSUNG CHANGChang LMa H.-KHuang C.-C.2021-08-032021-08-0320202050750Xhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85084192250&doi=10.1039%2fc9tb02468a&partnerID=40&md5=8ea501b57a3560948c3a697713fbe6b3https://scholars.lib.ntu.edu.tw/handle/123456789/575759In this study, we have developed a rapid and cost-effective method employing platinum ion (Pt4+)-capped fluorescent carbon quantum dots (CQDs) coupled with loop-mediated isothermal amplification (LAMP) to detect dual MRSA genes. We synthesized nitrogen- and chlorine-co-doped fluorescent CQDs (CQDSPDs) from spermidine trihydrochlorideviaa simple one-step pyrolysis. The CQDSPDscapped with Pt4+ions through the cooperative coordination of the amine and chlorine groups on the surface of CQDs facilitated the double-stranded DNA (dsDNA)-induced fluorescence quenching of CQDs, and enabled the construction of the CQDSPDs/Pt4+probe for the detection of as few as 10 copies of the MRSA gene (mecAandfemA). The sensitivity and specificity of the CQDSPDs/Pt4+probe for MRSA detection in clinical specimens (n= 24) were 94% and 86%, respectively. Our results reveal that the CQDSPDs/Pt4+probe has great potential for the diagnosis of antibiotic-resistant superbugs with high sensitivity, specificity, and agreement. ? The Royal Society of Chemistry 2019.Carbon; Chlorine; Cost effectiveness; Fluorescence; Genes; Ions; Nanocrystals; Platinum; Probes; Quenching; Semiconductor quantum dots; Co-doped; Cost-effective methods; Double-stranded DNA (ds-DNA); High sensitivity; Loop mediated isothermal amplifications; Platinum ions; Sensitivity and specificity; Spermidine; Carbon Quantum Dots; carbon; DNA; fluorescent dye; ion; platinum; quantum dot; chemical structure; chemistry; genetics; human; isolation and purification; methicillin resistant Staphylococcus aureus; particle size; Staphylococcus infection; surface property; Carbon; DNA; Fluorescent Dyes; Humans; Ions; Methicillin-Resistant Staphylococcus aureus; Molecular Structure; Particle Size; Platinum; Quantum Dots; Staphylococcal Infections; Surface Propertiesjournal article10.1039/c9tb02468a318593312-s2.0-85084192250