2006-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/683796摘要：土肉桂 (Cinnamomum osmophloeum Kaneh.) 是臺灣固有的闊葉樹種。至目前為止已有相當多篇的研究指出土肉桂之二次代謝產物 (抽出成分)對於細菌、黴菌、蟻及&#34728;都有不錯的抑制效果。但就分子生物學的角度來看，這些參與土肉桂生長、發育及二次代謝物的生合成相關酵素研究，則尚未有研究論文發表。因此本計畫即欲進行土肉桂功能性基因體之研究，利用基因庫cDNA library建構，匯集性狀表現基因（Expressed Sequence Tags，EST），製備DNA 晶片；之後，我們將可收集收集不同樹齡、不同品系、不同生育地、其他不同環境因子 (如溫、濕度、及光線等逆境處理) 的土肉桂材料、或組織培養苗，進行核酸雜交的反應，分析整體基因表現的情形，如此將可快速找出哪些基因可能與土肉桂生長發育或生理反應有關。我們希望藉此研究策略，能在短時間內釐清與土肉桂生長分化與二次代謝物生合成有關之酵素，以提供未來從事樹木生理、演化、基因轉殖、及藥用成分開發等研究時之重要參考資料。<br> Abstract: Cinnamomum osmophloeum Kaneh. is a native tree in Taiwan. There are several papers dealing with the antibacteria, antifungal, antitermite, antiant, and antitumor activities of the extractives isolated from Cinnamomum osmophloeum. However, investigation of the importance of enzymes in the plant development has been hampered by a lack of information at a molecular level. Therefore, in this proposed project, we will study on the functional genomics of Cinnamomum osmophloeum. Firstly, we will construction the cDNA libraries of Cinnamomum osmophloeum. Collection the expression sequence tags (ESTs) to be spotted on the DNA microrray. Using hybridization to analysis the gene expression at different aged, lines, growth area, and environmental factors (ex temperature, humidity, and light), rapid screening the genes involved in Cinnamomum osmophloeum development or physiological response. These will obtain the genes associated with differential and metabolism to provide the useful information for the researches on tree physiology, evolution, gene transformation, and drug discovery in the future.土肉桂生物晶片基因表現微體繁殖Cinnamomum osmophloeumMicroarrayGene expressionMicropropagation