2011-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/658482摘要：分子影像的的多重應用及活體標的追蹤，是極受重視的新穎性奈米生物科技。近來許多奈米結構紛紛被合成，影像標的的開發也日新月異；然而，設計出高解析度、靈敏度，且能夠直接進行活體檢測的影像系統，仍極具挑戰。目前，奈米材料表面的功能修飾，可使其生物相容性增加，並具備活體內被操控的能力。最快速的脂質自發包覆於金屬或非金屬粒子表面為修飾的有效方式。我們利用實驗室自行合成的新穎性正價膽固醇脂質GEC-Chol，並利用超音波震盪和粒子高壓擠壓成形法，開發出快速脂質奈米粒子賦型技術，理論上，這些脂質奈米粒子可以具有同時包覆基因、藥物、無機奈米粒子等的特性。這些藉由脂質外層修飾的PEG分子上連接標靶配體，產生導向性之多功能奈米粒子。進一步發現此種奈米粒子不僅具有原先之物性化性，並具有高訊噪比之非線性光學特性，這些脂質外層和內含的無機粒子亦能交互產生新穎的物理特性，在細胞或動物模型的分子影像研究深具潛力。這些多功能奈米載體，以脂質氧化鐵粒子為例，它能用外加磁場來增強遞送效率，並利用三倍頻顯微術作高解析度影像分析，在大體影像上能用於核磁共振顯影，甚或將來應用於活體之熱炙治療。因此多功能的脂質奈米粒子，在藥物遞送、多重影像分析及新穎性治療系統的共同搭配下，對未來的奈米醫學有相當的衝擊。<br> Abstract: Molecular imaging is an emerging field in functional diagnosis and nanomedicine. Manynovel nanostructures were synthesized and characterized for imaging or therapeutic needs.However, the designing of good imaging modality with high sensitivity and resolution formonitoring important phenomena in living subject is still challenging. For the time being,surface modification of nanomaterial is the first step to designs such biocompatiblenanoparticles with controlled ability in vivo. The feasible approach to render a surfacebiocompatible is the spontaneous deposition of lipid membrane onto the solid surface of suchnanomaterials. For the past few years, we have developed a simple yet versatile formulationof nanosized particles encapsulating nanodiamonds, iron oxide nanoparticles, quantum dots,silica nanoparticles. We further discovered these core-shell nanostructures not only have thefluorescent or resonance properties, but also have the strongest signals in the non-linearoptical imaging. For example, the iron oxide moiety helps the magnet-enhanced nanoparticledelivery, third harmonic generation (THG) microscopic analysis, MRI imaging, as well as forpotential hyperthermia therapy. With the combination selection of different nanoparticles inthe core, multifunctional properties of such lipid-based core-shell structures could be achieved.Further, their potential physic properties will be analyzed in the in vivo environment. Themultifunctional nanoparticles with targeted properties can also be achieved by adding targetedligands onto the PEG linker of the lipid coat. Such development of multifunctionallipid-nanoparticles for multimodality imaging and therapy is going to have a good impact onnanomedicine field.核磁共振顯影超順磁性氧化鐵奈米粒子倍頻顯微術小鼠耳朵腫瘤模式Magnetic resonance imagingiron oxide nanoparticlesharmonic generation microscopymouse ear tumor model