Hybridization Protection Reaction for Sensitive and Robust Gene Expression Profiling of Clinical Formalin-Fixed Paraffin-Embedded Samples

FENG-MING HSUYIH-LEONG CHANGChen, Chung-YungChung-YungChenLin, Shu-RungShu-RungLinCHIA-HSIEN CHENG2023-12-152023-12-152023-12-010009-91471530-8561https://scholars.lib.ntu.edu.tw/handle/123456789/637874RNA profiling of formalin-fixed paraffin-embedded (FFPE) tumor tissues for the molecular diagnostics of disease prognosis or treatment response is often irreproducible and limited to a handful of biomarkers. This has led to an unmet need for robust multiplexed assays that can profile several RNA biomarkers of interest using a limited amount of specimen. Here, we describe hybridization protection reaction (HPR), which is a novel RNA profiling approach with high reproducibility.en[SDGs]SDG3Hybridization Protection Reaction for Sensitive and Robust Gene Expression Profiling of Clinical Formalin-Fixed Paraffin-Embedded Samplesjournal article10.1093/clinchem/hvad170379644182-s2.0-85178651457https://api.elsevier.com/content/abstract/scopus_id/85178651457