MING-JIUM SHIEHShieh Y.A.Lai P.S.2020-02-272020-02-272009https://www.scopus.com/inward/record.uri?eid=2-s2.0-77950830690&doi=10.1109%2fICBPE.2009.5384070&partnerID=40&md5=eac1d2376de33c5e2131c9b58a32eec2https://scholars.lib.ntu.edu.tw/handle/123456789/465792A specialized G-quadruplex DNA aptamer with nucleolin targeting ability, AS1411, has been studied for cancer therapy. In this study, we report a novel delivery strategy for chemo-photodynamic combined treatment using AS1411 aptamer conjugated with tetra-(N-methyl-4-pyridyl)-porphine by intercalation and outside binding. Our results show that the apt-TMP complex exhibited higher TMPyP4 accumulation in nucleolin over-expressing MCF-7 breast cancer cells than in M10 normal epithelium cells. After irradiation for 180 sec, the photodamage was apparently observed in MCF-7 cells. These results indicate that AS1411 aptamer can play as a potential photosensitizer carrier for for cancer therapy. ?2009 IEEE.[SDGs]SDG3Aptamers; Cancer therapy; Combined treatment; Epithelium cells; G-quadruplexes; MCF-7 breast cancer cells; MCF-7 cells; Nucleolin; Outside binding; Photo-damage; Porphine; Pyridyl; Oncology; Photosensitizersconference paper10.1109/ICBPE.2009.53840702-s2.0-77950830690