PEI-JEN LOUWEN-PIN CHENCHIN-TARNG LINChen H.-C.Wu J.-C.2020-10-272020-10-2720000730-2312https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033788821&doi=10.1002%2f1097-4644%2820001215%2979%3a4%3c542%3a%3aAID-JCB30%3e3.0.CO%3b2-Q&partnerID=40&md5=77db5fc38774ba1a91bfd88d68475c9dhttps://scholars.lib.ntu.edu.tw/handle/123456789/518330Taxol affects microtubule dynamics by promoting microtubule assembly. To obtain a better insight into possible cross-talk between the microtubule- and actin-based cytoskeletons, we studied the short-term effects of Taxol treatment on the expression of actin and the E-cadherin/catenin complex in the nasopharyngeal carcinoma cell line TW-039 using immunofluorescence, immunoprecipitation, and immunoblotting methods. Morphologic changes in actin filaments, including ventral actin dumps and perijunctional actin blebs, were seen at Taxol concentrations ?1 μM. Levels of detergent-soluble E-cadherin fell to 53% or 58% compared to controls in cells treated, respectively, with 1 or 5 μM Taxol, while levels of detergent-soluble β-catenin fell to 76% or 74%. Levels of the detergent-soluble pool of α- and γ-catenin and the detergent-insoluble pool of the E-cadherin/catenin complex were unchanged by Taxol treatment and no significant difference was seen in the levels of adenomatous polyposis coli or glycogen synthase-3β or tyrosine phosphorylation patterns. These results suggest that modulation of microtubule dynamics by Taxol may have effects on the expression of actin and the cytosolic E-cadherin and β-catenin in nasopharyngeal carcinoma cells through pathways not involving the phosphorylation of β-catenin. (C) 2000 Wiley-Liss, Inc.[SDGs]SDG3actin; beta catenin; paclitaxel; uvomorulin; actin filament; article; cancer cell culture; controlled study; cytosol; drug effect; microtubule assembly; molecular interaction; nasopharynx carcinoma; priority journal; protein phosphorylation; Actins; Adenomatous Polyposis Coli Protein; Antineoplastic Agents, Phytogenic; Ca(2+)-Calmodulin Dependent Protein Kinase; Cadherins; Cell Size; Cytoskeletal Proteins; Cytoskeleton; Cytosol; Human; Microtubules; Nasopharyngeal Neoplasms; Neoplasm Proteins; Paclitaxel; Phosphorylation; Support, Non-U.S. Gov't; Tumor Cells, Cultured; Tyrosinejournal article10.1002/1097-4644(20001215)79:4<542109968452-s2.0-0033788821