KAI-YIN LOSun, Yung ShinYung ShinSunLin, Jin YoungJin YoungLin2019-10-022019-10-022019-01-012019https://scholars.lib.ntu.edu.tw/handle/123456789/425793© 2019 by the authors. The wound-healing assay is commonly and widely used for investigating collective cell migration under various physical and chemical stimuli. Substrate-coating materials are shown to affect the wound-healing process in a cell-type dependent manner. However, experiment-to-experiment variations make it difficult to compare results from different assays. In this paper, a modified barrier wound-healing assay was reported for studying the wound-healing process on different substrates in one single petri dish. In short, half of a dish was covered with the tape, and coating materials, poly-l-lysine and gelatin, were applied to the surface. After peeling off the tape, half of the surface was coated with the desired material. Then a customized barrier was placed inside the dish to create the wound. The results indicated that surface coating did not affect cell proliferation/viability, and the wound-healing rate increased in coated surfaces compared to uncoated ones. The present study provides a platform for further understanding the mechanisms of substrate coating-dependent wound-healing processes.Cell migration | Fibroblasts | Surface coating | Wound-healing assay | Wound-healing ratejournal article10.3390/ma121727752-s2.0-85071842677https://api.elsevier.com/content/abstract/scopus_id/85071842677