Expression of angiopoietins and vascular endothelial growth factors and their clinical significance in acute myeloid leukemia

HSIN-AN HOUWEN-CHIEN CHOULIANG-IN LINJIH-LUH TANGTseng M.-H.Huang C.-F.MING YAOChen, Chien-YuanChien-YuanChenWOEI TSAYHWEI-FANG TIEN2020-06-162020-06-1620080145-2126https://scholars.lib.ntu.edu.tw/handle/123456789/502706Angiogenic factors play an essential role in normal and pathologic angiogenesis, but their clinical role in acute myeloid leukemia (AML) remains unclear. We investigated the expression of Ang-1, Ang-2, Tie2, VEGF-A, and VEGF-C genes in bone marrow (BM) mononuclear cells by real-time quantitative PCR (RQ-PCR) in a cohort of 126 patients with newly diagnosed de novo AML and normal marrow donors. Here we show that high pre-treatment levels of Ang-2 in the BM indicate an unfavorable prognosis in AML. Only karyotype (hazard ratio 2.19, 95% CI 1.25-3.42, P = 0.005) and expression of Ang-2 (hazard ratio 2.05, 95% CI 1.20-3.52, P = 0.009), but not other angiogenic factors, were independent prognostic factors for overall survival by multivariate analysis. The prognostic significance of Ang-2 expression was more obvious in the subgroup of patients with intermediate-risk cytogenetics. Subgroup analysis showed that Ang-2 expression had prognostic impact on patients with low (but not high) Ang-1 or Tie2 levels, and on patients with high (but not low) VEGF-A or VEGF-C levels. ? 2007 Elsevier Ltd. All rights reserved.[SDGs]SDG3angiopoietin 1; angiopoietin 2; angiopoietin receptor; cytarabine; daunorubicin; idarubicin; vasculotropin A; vasculotropin C; acute granulocytic leukemia; adolescent; adult; aged; article; cancer chemotherapy; clinical feature; combination chemotherapy; controlled study; cytogenetics; drug megadose; female; human; human cell; karyotype; major clinical study; male; monotherapy; outcome assessment; overall survival; priority journal; prognosis; protein expression; reverse transcription polymerase chain reaction; Adolescent; Adult; Aged; Aged, 80 and over; Angiopoietin-1; Angiopoietin-2; Bone Marrow; Cohort Studies; DNA Primers; Enzyme-Linked Immunosorbent Assay; Female; Gene Expression Regulation, Leukemic; Humans; Immunoenzyme Techniques; Leukemia, Myeloid, Acute; Male; Middle Aged; Prognosis; Receptor, TIE-2; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Survival Rate; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor CExpression of angiopoietins and vascular endothelial growth factors and their clinical significance in acute myeloid leukemiajournal article10.1016/j.leukres.2007.08.010179046342-s2.0-40849088142