2020-10-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/653330Pathogens, referring to infectious microorganism or agent, such as a virus, bacterium, fungus, etc., evoke an immune response and cause disease or even death to a host. Among all types of pathogens, pathogenetic bacterium and virus such as tuberculosis (TB) and human immunodeficiency virus (HIV) thread human beings the most due to their high transmissibility, pathogenicity, and fatality. According to the annual report of the World Health Organization, TB and HIV caused an estimated 1.2 million and 0.77 million deaths in 2018, respectively. To effectively control the wide spreading of the pathogenetic bacterium and virus and inhibit human beings from infectious diseases, real-time detection is crucially needed. To achieve real-time detection, loop-mediate isothermal amplification is developed and integrated into a portable microfluidic device to identify and quantify the pathogenetic bacterium and virus in blood samples. However, the current devices still consume a long time for the detection and can only process one sample or one target in each test, leading to the futility of the devices in processing real-world samples which are normally in large quantities and contain multiple targets. In this proposal, we propose a highly portable pathogenetic bacterium and virus detector to address the above-mentioned problems. The proposed device can provide key functions, including (1) extracting nucleic acids directly from blood and (2) detecting the extracted nucleic acids with ultra-high sensitivity based on (3) loop-mediated isothermal amplification technique using high sensitive nanoprobes on the lateral flow immunoassay strips. Besides, the device enables untrained personnel to achieve the detection result by integrating the device with the (4) photoelectronic components and (5) artificial intelligence. Benefiting from these key functions, we believe that the proposed portable device can achieve the goals of easy-operation, high sensitivity, and multi-target detection, and contribute to clinical diagnosis and pathogen control, protecting human beings from infectious diseases.pathogen detection; microfluidics; portable detector; nucleic acid amplification; nanoprobe