Fan-Jiang P.-YLee P.-SNagabhushanam KHo C.-TMIN-HSIUNG PAN2022-04-252022-04-25202100218561https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110243208&doi=10.1021%2facs.jafc.1c02783&partnerID=40&md5=a53002f489ab492ff45582a03b5217e1https://scholars.lib.ntu.edu.tw/handle/123456789/606141The worldwide prevalence of obesity has significantly increased over the past few decades. It is currently believed that obesity is a risk factor for developing inflammatory bowel disease. Pterostilbene (PTS), a naturally occurring stilbene from blueberries, is known to have anticancer, anti-inflammation, antifibrosis, and antiobesity effects. The preventive effect of PTS on the susceptibility of high-fat diet (HFD) to dextran sulfate sodium (DSS)-induced colitis in mice was investigated. Beginning at 5 weeks of age, C57BL/6J mice were fed a normal diet, 50% HFD alone, or containing PTS, and DSS (2.5%, w/v) was given in drinking water at week 9 and week 11. The results demonstrated that PTS significantly attenuated HFD and DSS-induced plasma interleukin-6 accumulation. Moreover, PTS suppressed HFD/DSS-induced formation of aberrant crypt foci and reduced the colon weight-to-length ratio in HFD/DSS-induced colitis mice. Furthermore, PTS inhibited interleukin-1β (IL-1β), the C/EBP homologous protein (CHOP), cyclooxygenase-2, and transforming growth factor beta-1 (TGF-β1)/mothers against decapentaplegic homolog 2 expression and maintained mucin2 (Muc2) and E-cadherin expressions. In addition, post-treatment with PTS also decreased the colon weight-to-length ratio and loss of Muc2. Moreover, the CHOP, IL-1β, matrix metalloproteinase-2, and TGF-β1 expressions were significantly decreased in HFD/DSS-induced colitis mice after post-treatment with PTS. In conclusion, the results of the present study suggest that PTS is of significant interest for the prevention of HFD/DSS-induced colitis in C57BL/6J mice. ?colitisdextran sulfate sodium (DSS)high-fat diet (HFD)intestinal fibrosispterostilbene (PTS)DextranMammalsNutritionPathologyPotable waterSodiumSulfur compoundsDextran sulfate sodiumDextran sulfate sodium (DSS) induced colitisE-cadherin expressionHomologous proteinsInflammatory bowel diseaseMatrix metalloproteinase-2Naturally occurringTransforming growth factor betaDiseasesdextran sulfategelatinase Apterostilbenestilbene derivativeadverse eventanimalC57BL mousecolondisease modelfibrosisgeneticsinflammationlipid dietmousepathologyAnimalsColitisColonDextran SulfateDiet, High-FatDisease Models, AnimalFibrosisInflammationMatrix Metalloproteinase 2MiceMice, Inbred C57BLStilbenes[SDGs]SDG3journal article10.1021/acs.jafc.1c02783341521362-s2.0-85110243208