SANG-HENG KOKJANG-JAER LEEHsu H.-C.CHUN-PIN CHIANGKuo Y.-S.YEN-PING KUO2021-07-052021-07-0520020904-2512https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036688271&doi=10.1034%2fj.1600-0714.2002.00118.x&partnerID=40&md5=20429682ad846379d28b376aeacb9bb8https://scholars.lib.ntu.edu.tw/handle/123456789/569213Background: Adenomatous polyposis coli (APC) gene mutations have been demonstrated not only in colorectal tumors but also in a variety of human cancers. Methods: To elucidate the possible roles of APC gene mutations in oral squamous cell carcinomas (OSCCs), we examined 40 untreated human primary OSCCs using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing assays. Results: By screening nearly one-half of the coding region (codons 279-1673, including the MCR) of the APC gene, five missense mutations and a 1-base pair deletion were detected in five (12.5%) tumors, resulting in five amino-acid substitutions or a truncation of the APC protein. All patients with APC mutations were both areca quid chewers and tobacco smokers (P = 0.049). Conclusions: These results suggest that APC mutations may also contribute to the carcinogenesis of at least some OSCCs in Taiwan, especially for the users of areca quid and tobacco.[SDGs]SDG3Adenomatous Polyposis Coli Protein; Adult; Aged; Aged, 80 and over; Amino Acid Substitution; Areca; Base Pairing; Carcinoma, Squamous Cell; Chi-Square Distribution; Codon; Exons; Female; Frameshift Mutation; Gene Deletion; Genes, APC; Humans; Loss of Heterozygosity; Male; Middle Aged; Mouth Neoplasms; Mutation; Mutation, Missense; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Sequence Analysis, DNA; Smoking; Statistics; TaiwanMutations of the adenomatous polyposis coli gene in areca quid and tobacco-associated oral squamous cell carcinomas in Taiwanjournal article10.1034/j.1600-0714.2002.00118.x121650572-s2.0-0036688271