2016-02-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/645108摘要：肝癌為相當惡性之腫瘤，每年全世界有超過 700,000 名病人被確診罹患肝癌，多數患者臨床上並無顯著早期徵兆，因而就醫診斷時可能皆已肝癌晚期，不僅肝功能狀況不佳使得治療手段的選擇有所侷限，亦降低了腫瘤之治療有效性，五年存活率僅10%，因此發展一個有效的肝癌治療方法有迫切的需要。超音波對比劑為一種可被生物分解的微氣泡，具有增強聲場散射信號之作用，超音波影像技術隨著超音波對比劑的發展改善了成像的空間解析度及影像動態範圍。在臨床應用上除了傳統心血管疾病與癌症之診斷，近來更被應用於促進藥物釋放與提升化學治療效率上。博信生物科技之技術提供了一安全有效之微氣泡平台，相較目前國際產品提供了更小的微氣泡粒徑與最佳化之濃度，能在較低的注射劑量下有效提升產品之效價與影像解析度，且能減少慣性穴蝕的發生，增加使用之安全性。本計畫預計提出肝癌治療之新治療策略，以博信生物科技公司所研發之微氣泡與治療用超音波系統，藉由超音波與超音波對比劑間的交互作用，提升局部腫瘤區域之通透性，同時降低全身性之化療毒性，將此技術應用於小動物肝腫瘤之治療，預期：(1)提升傳統化療舊藥之效率；(2)合併肝腫瘤燒灼治療，設計有效之新式肝癌治療流程。<br> Abstract: Hepatocellular carcinoma (HCC) is a malignant cancer and the third leading cause ofcancer mortality worldwide. The majority of patients experience no significant early signs,and thus once the diagnosis of HCC is confirmed, course of the disease has entered the latestage. The poor liver function and the advanced disease hinder the selection of treatmentoptions and lower the efficiency of cancer treatment, contributing to a relatively lowreported 5-year survival rate of approximately 10%.Ultrasound contrast agents (UCAs), as biodegradable microbubbles, have the abilityof enhancing ultrasound scattering signals. The development of UCAs improves the spatialresolution and dynamic range of ultrasound imaging techniques. Conventional clinicalapplications include diagnosis of cardiovascular diseases and tumor diagnosis. Recently,UCAs are applied to enhace the drug delivery. Trust Bio-sononics, Inc. provides a safe andeffective microbubble platform (USpheresTM) which possesses smaller size distribution andmore optimized concentration compared to the current commercialized UCAs, enhancing thepotency and image resolution under lower given dose. Furthermore, the small size ofUSpheresTM series reduces the inertial cavitation, improving in vivo safety of UCAs.The aim of this study is to propose a new HCC treatment strategy by using the USpheresTMmicrobubbles and ultrasound treatment system estabilished by Bio-sononics, Inc. Theinteractions of ultrasound and microbubbles enhance the local permeability of tumor, andreduce the systematic toxicity arised from chemotherapy. Applying this technique on smallanimal HCC model, it is anticipated to improve the treatment efficiency of the currentclinically used chemotherapeutic drugs. Futhermore, combining with radio-frequencyablation, a new and efficient HCC treatment strategy is designed.超音波超音波對比劑肝癌治療化學治療肝癌射頻燒灼技術ultrasoundultrasound contrast agentsHCC treatmentchemotherapyradio-frequency ablation