Wang, C.-H.C.-H.WangChiou, S.-H.S.-H.ChiouChou, C.-P.C.-P.ChouChen, Y.-C.Y.-C.ChenHuang, Y.-J.Y.-J.HuangPeng, C.-A.C.-A.Peng2019-05-092019-05-092011https://scholars.lib.ntu.edu.tw/handle/123456789/407002CD133+ cells in glioblastoma (GBM) display cancer stem cell-like properties and have been considered as the culprit of tumor recurrence, justifying exploration of potential therapeutic modalities targeting CD133+ cancer stem-like cells (CSCs). For photothermolysis studies, GBM-CD133+ and GBM-CD133- cells mixed with various ratios were challenged with single-walled carbon nanotubes (SWNTs) conjugated with CD133 monoclonal antibody (anti-CD133) and then irradiated with near-infrared laser light. Results show that GBM-CD133+ cells were selectively targeted and eradicated, whereas GBM-CD133- cells remained viable. In addition, in vitro tumorigenic and self-renewal capability of GBM-CD133+ treated with localized hyperthermia was significantly blocked. Furthermore, GBM-CD133+ cells pretreated with anti-CD133-SWNTs and irradiated by near-infrared laser 2 days after xenotransplantation in nude mice did not exhibit sustainability of CSC features for tumor growth. Taken altogether, our studies demonstrated that anti-CD133-SWNTs have the potential to be utilized as a thermal-coupling agent to effectively target and destroy GBM CSCs in vitro and in vivo. From the Clinical Editor: Glioblastoma remains one of the most notorious cancer from the standpoint of recurrence and overall resistance to therapy. CD133+ stem cells occur among GBM cells, and may be responsible for the huge recurrence risk. This paper discusses a targeted elimination method of these cells, which may enable more efficient therapy in an effort to minimize or prevent recurrence. ? 2011 Elsevier Inc.Cancer stem-like cells; CD133; Glioblastoma; Near-infrared laser; Single-walled carbon nanotubes[SDGs]SDG3Cancer stem-like cells; CD133; Glioblastomas; Near-infrared lasers; Single-walled carbon; Coupling agents; Cryogenic liquids; Cytology; Infrared devices; Laser surgery; Monoclonal antibodies; Single-walled carbon nanotubes (SWCN); Stem cells; Surface plasmon resonance; Tumors; Infrared lasers; antibody conjugate; CD133 antigen; monoclonal antibody; monoclonal antibody CD133; single walled nanotube; unclassified drug; animal experiment; animal model; article; biological therapy; cancer growth; cancer stem cell; cell renewal; cell viability; controlled study; endocytosis; gene expression; glioblastoma; glioblastoma stem like cell; human; human cell; hyperthermic therapy; laser; molecularly targeted therapy; mouse; nonhuman; photolysis; photothermolysis; xenotransplantation; Animals; Antibodies, Monoclonal; Antigens, CD; Glioblastoma; Glycoproteins; Humans; Hyperthermia, Induced; Mice; Mice, Nude; Nanotubes, Carbon; Neoplastic Stem Cells; Peptides; Tumor Cells, Cultured; Mus musculusjournal article10.1016/j.nano.2010.06.0102-s2.0-78751702579