2009-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/661767摘要：葵百合LsGPP1為以差異性PCR所選殖之水楊酸誘導性基因，過去研究結果顯示以 水楊酸、撲殺熱與百合灰黴病菌Botrytis elliptica 處理葵百合均可誘導LsGPP1基因 表現，可能為一深具發展潛力之抗性基因。本研究欲藉由農桿菌注入法表現 LsGPP1啟動子:GUS基因重組雙向表現載體，以及建構表現LsGPP1啟動子:GUS基 因之阿拉伯芥轉型株兩策略，探討LsGPP1之表現調控及其啟動子之特性以及其啟 動子之特性與應用價值。本年度之目標為確定LsGPP1啟動子(1) 於不同組織、器 官、生育階段之表現性質，(2) 受到各種生物及非生物性逆境、植物荷爾蒙以及訊 息傳遞路經相關分子刺激之表現特徵，以及(3)在不同植物物種之表現程度，並希 望藉此研究為LsGPP1啟動子提供在植物病害防治與其他生物科技範圍應用之基礎<br> Abstract: For the analysis of the mode of gene expression and the application in molecular breeding of disease resistance, the promoter of the salicylic acid and Botrytis ellipticainducible gene-LsGRP1 was cloned and analysis its expression traits. For the analysis of LsGRP1 promoter expression profile, the activity assay of the GUS gene driven by LsGRP1 promoter will be detected in a agroinfiltration assay and in a Arabidopsis transformant. There are three aims in this year: (1) To measure the activity of LsGRP1 promoter in different tissues, organs and developmental stages. (2) To detect the stress responses of LsGRP1 promoter under the treatments of abiotic/biotic factors, signaling molecule and plant hormones. (3) To identify the expression level of LsGRP1 promoter in different kinds of plant species. This study will provide the information about the usage of LsGRP1 promoter in molecular breeding and plant disease control.啟動子病原誘導性單子葉植物分子育種promoterpathogen-induciblemonocotyledonmolecular breeding