YUAN-LING LEEHong C.-Y.SANG-HENG KOKHou K.-L.Lin Y.-T.Chen M.-H.Wang C.-C.SZE-KWAN LIN2021-07-052021-07-0520090099-2399https://www.scopus.com/inward/record.uri?eid=2-s2.0-58249116962&doi=10.1016%2fj.joen.2008.11.015&partnerID=40&md5=41fdd67069f8ca161a546bbbae9a374dhttps://scholars.lib.ntu.edu.tw/handle/123456789/569403Recent investigations indicate that epigallocatechin-3-gallate (EGCG), the major polyphenol of green tea, has anti-inflammatory properties. This study assessed the effect of EGCG on oncostatin M (OSM)-induced synthesis of cysteine-rich 61 (Cyr61), a potential osteolytic mediator, in MG-63 human osteoblastic cells. The therapeutic effect of EGCG in apical periodontitis in rats was also examined. Western blot analysis showed that OSM stimulated Cyr61 synthesis in MG-63 in a time-dependent manner, whereas EGCG readily attenuated this effect. On the other hand, Cyr61 treatment of MG-63 cells induced the release of CCL2, a chemokine responsible for macrophage chemotaxis. In a rat model of induced apical periodontitis, radiography and histopathology revealed that administration of EGCG markedly diminished the severity of periapical lesions. The numbers of Cyr61-synthesizing osteoblasts and infiltrating macrophages were also decreased. Thus, EGCG suppresses the progression of apical periodontitis, possibly by diminishing Cyr61 expression in osteoblasts and, subsequently, macrophage chemotaxis into the lesions. ? 2008 American Association of Endodontists.[SDGs]SDG3antioxidant; catechin; cysteine rich protein 61; drug derivative; epigallocatechin gallate; monocyte chemotactic protein 1; oncostatin M; plant extract; animal; article; biosynthesis; chemistry; disease model; drug antagonism; drug effect; human; metabolism; neutrophil chemotaxis; osteoblast; osteolysis; rat; tea; tooth periapical disease; tumor cell line; Wistar rat; Alveolar Bone Loss; Animals; Antioxidants; Catechin; Cell Line, Tumor; Chemokine CCL2; Chemotaxis, Leukocyte; Cysteine-Rich Protein 61; Disease Models, Animal; Humans; Oncostatin M; Osteoblasts; Periapical Periodontitis; Plant Extracts; Rats; Rats, Wistar; Teajournal article10.1016/j.joen.2008.11.015191667742-s2.0-58249116962