2012-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/657722摘要：過去有關幹細胞分生的研究主要著重在奈米顆粒對脂肪分化，軟骨細胞分化，骨質細胞分化的能力影響程度。但多年來不同論文中常有不同的結論，近年來也陸續出現論文結果顯示MR磁場對軟骨細胞的生長有嚴重影響。而細胞治療神經系統疾病已成為一種趨勢及方向。但對磁性奈米顆粒及磁場的影響下，幹細胞分 生成神經細胞的能力及影響程度的研究都十分缺乏。本研究分成兩年，第一年著重在研究磁場對幹細胞分生成神經細胞的能力的影響。第二年乃使用奈米磁性顆粒標誌幹細胞後，再進行相同分生成神經細胞的能力研究。以確認這兩者對幹細胞有關神經分生的能力的影響，以供日後作細胞治療時，作細胞追蹤術時的重要參考依據。<br> Abstract: Most of the previous studies about nano-magnetic particles regarding to the stem cell differentiation were focusing on the effect to adipogenic, chondrogenic, and osteogenic differentiation capability. However, the conclusion is not quite unique and depending on different center and conditions. Recently, some papers also mentioned that MR magnetic field had negative effect to the growth of chondrocytes. Cell therapy become one of the main trend for the treatment of central nervous system disorders.However, there is a lack of result to show what is the effect of the nano-magnetic particles and magnetic field to the stem cell-derived neurons.This is a 2-year research. In the 1st year of this study, we have to perform the process to induce the neuronal differentiation of MSC. After confirmation of morphology with conventional H&E stain, we shall perform characterization of the stem cell-derived neuronal differentiation with immunofluorescence staining, flow cytometry, RT-PCR, and western blot with and without magnetic field.In the 2nd year of this study, we repeat the same study with SPIO (Resovist) labeling MSC. So comparison of this study with the one done on 1st year, we can understand the effect of SPIO on the neuronal differentiation capacity of MSC with and without magnetic field. Finally we shall examine labeling neuron with MR system to see if we can detect the neuron with MR technique in vitro.磁場幹細胞奈米顆粒細胞治療分生Magnetic fieldstem cellnanoparticlecellular therapydifferentiation