Su J.-HLiu C.-ILu M.-CChang C.-IHsieh M.-YLin Y.-CDai C.-FZhang Y.-HLin Z.-YCHANG-FENG DAI2021-07-172021-07-17202114786419https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070269867&doi=10.1080%2f14786419.2019.1614579&partnerID=40&md5=9fba8f049a55c331443564e2d45eb0echttps://scholars.lib.ntu.edu.tw/handle/123456789/571831We have conducted a long-term research on the Taiwanese soft coral Asterospicularia laurae, which resulted in many xenicane-type diterpenoids such as asterolaurins A–M from A. laurae coral tissues during the non-spawning period were isolated. Here, we report a new xenicane diterpenoid, asterolaurin N (1), along with three known xenicane-type monocarbocyclic diterpenes [13-epi-9-desacetylxenicin (2), xeniolide-B 9-acetate (3) and asterolaurin I (4)] from A. laurae during the spawning period. The structures of the new secondary metabolite were established with an extensive spectroscopic analysis. The 1D and 2D nuclear magnetic resonance (NMR) data of the compounds were discussed. We discovered that the C-15 of 1 contains two methyl groups on a carbon bearing an acetyl group, which has not been reported previously. In addition, Compounds 1, 3, and 4 showed selective cytotoxic activity against Molt 4, while 2 exhibited significant cytotoxicity against Molt 4, K562, Sup-T1 and U937 cell lines. ? 2019 Informa UK Limited, trading as Taylor & Francis Group.13 epi 9 desacetylxenicin; asterolaurin I; asterolaurin N; cytotoxic agent; diterpenoid; doxorubicin; unclassified drug; xeniolide B 9 acetic acid; antineoplastic agent; diterpenoid; Article; Asterospicularia laurae; coral; crystal structure; cytotoxicity; drug structure; drug synthesis; IC50; K-562 cell line; nonhuman; proton nuclear magnetic resonance; SUP-T1 cell line; Taiwan; U-937 cell line; animal; Anthozoa; carbon nuclear magnetic resonance; cell death; chemistry; drug effect; drug screening; human; metabolism; secondary metabolism; tumor cell line; Animals; Anthozoa; Antineoplastic Agents; Carbon-13 Magnetic Resonance Spectroscopy; Cell Death; Cell Line, Tumor; Diterpenes; Drug Screening Assays, Antitumor; Humans; Inhibitory Concentration 50; Proton Magnetic Resonance Spectroscopy; Secondary Metabolism; Taiwan[SDGs]SDG3[SDGs]SDG14journal article10.1080/14786419.2019.1614579313648812-s2.0-85070269867