Yap K.L.Hafez M.J.TSUI-LIEN MAOKurman R.J.Murphy K.M.Shih I.-M.2020-03-072020-03-0720101687-8450https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930533354&doi=10.1155%2f2010%2f364508&partnerID=40&md5=1f62d415f0ec619af4b424a830a38ac6https://scholars.lib.ntu.edu.tw/handle/123456789/473639Gestational trophoblastic neoplasms (GTNs) are a rare group of neoplastic diseases composed of choriocarcinomas, placental site trophoblastic tumors (PSTTs) and epithelioid trophoblastic tumors (ETTs). Since these tumors are derivatives of fetal trophoblastic tissue, approximately 50% of GTN cases are expected to originate from a male conceptus and carry a Y-chromosomal complement according to a balanced sex ratio. To investigate this hypothesis, we carried out a comprehensive analysis by genotyping a relatively large sample size of 51 GTN cases using three independent sex chromosome genetic markers; Amelogenin, Protein Kinase and Zinc Finger have X and Y homologues that are distinguishable by their PCR product size. We found that all cases contained the X-chromosomal complement while only five (10%) of 51 tumors harbored the Y-chromosomal complement. Specifically, Y-chromosomal signals were detected in one (5%) of 19 choriocarcinomas, one (7%) of 15 PSTTs and three (18%) of 17 ETTs. The histopathological features of those with a Y-chromosome were similar to those without. Our results demonstrate the presence of a Y-chromosomal complement in GTNs, albeit a low 10% of cases. This shortfall of Y-chromosomal complements in GTNs may reinforce the notion that the majority of GTNs are derived from previous molar gestations. Copyright ? 2010 Kai Lee Yap et al.[SDGs]SDG3amelogenin; protein kinase; zinc finger protein; Article; choriocarcinoma; female; genetic marker; genotype; histopathology; human; human tissue; polymerase chain reaction; syncytiotrophoblast cell; trophoblast; trophoblastic tumor; X chromosome; Y chromosomejournal article10.1155/2010/3645082-s2.0-84930533354