2011-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/664833摘要：為避免基因轉殖植物的基因自田間試驗設施中外流，密閉溫室在整體規劃上除了同時應具備有結構上高度氣密性、區域性負壓以及進排氣口有效之過濾裝置。而根據前期研究對於國內田間試驗機構現場訪視結果發現，國內密閉溫室對於結構氣密要求並無太大問題，但區域性負壓設定以及濾網之選用之基準上，需要實務上以植物花粉溢散情境為測試對象進行系列測試以提供後續應用上之參考依據。根據過去兩年來對於田間試驗機構現場訪視結果所觀察之問題點，本研究建立測試系統以評估濾網及壓力分佈對於花粉逸散之影響，分別於足尺環境控制艙室中測試，並進一步於實場中驗證。 　　本研究共完成四種濾網對於微粒與常見溫網室基因轉殖作物花粉之過濾效能曲線，發展預測模式並證實可行，並完成實場驗證工作，其結果非常良好，可提供未來選用之參考。同時亦探討不同濾網組合之過濾效能比較，發現特定等級濾網組合除了過濾效能良好外，既節能且購置成本大幅降低。此外亦完成壓力分佈設定對阻絕 花粉逸散影響與成效評估，並發展花粉逸散指標以評估花粉在溫網室洩漏情形。在兼顧過濾效能與節能前提下，可利用此初步成果提供實場濾網選用之重要參考，以及阻絕花粉逸散最適化之壓力設定。<br> Abstract: Pollen dispersal plays an important role in the spread of genetically modified organisms (GMO). Therefore, the isolation performance of a sealed greenhouse which was determined by the air tightness, negative pressure control, and also equipped with proper filtration system, are three essential elements in practical applications. Except the air tightness, previous study shown that most facilities in Taiwan have the problems of inadequate setting of negative pressure control and also filter selections. Our study therefore aims to establish a system to assess the isolation performance by using filtration efficiency and the distribution of negative pressures in chamber study. Verification of the isolation performance in different facilities will also be conducted in our study. This study will use general particles and various pollens with different diameters as the test materials in chamber study. Filtration testing system that integrated with aerosols generation, particle counter, and pollen sampling system will be built to assess the real filtration efficiency for different pollen. Full scale chamber will also modified to simulate a distribution of negative pressure to evaluate the best parameters in preventing dispersion of pollen from sealed chamber. In this study, the pollen filtration curves of four different filters were obtained. The filtration predicted model of pollen was also developed and verified both in test chamber and field. The result can provide guidance for selecting different grades of filters based on the diameter of pollen or plants. The filtration efficiency of two filter combination was evaluated. Certain combinations of two filters were found has the same filtration efficiency as HEPA, and the cost is also relatively low. In addition, the effect and phenomenon of pollen leakage under different pressure distribution designs were investigated. A pollen leakage index was also developed to access and investigate the pollen leakage in practical applications of the sealed greenhouse.