2011-01-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/659260摘要：收集本省之養殖九孔&#30129;疹樣病毒，純化分析九孔&#30129;疹樣病毒之基因系列，並完成LAMP之引子對篩選。應用於病材之快速診斷，縮短檢查所需時間並可在現場應用，因此可及早偵測水產生物是否感染病毒，避免引入帶病毒之魚種，減少病毒爆發及疫情擴散。實施方法為採取九孔&#30129;疹樣病毒設計Outer primers、Inner primers和Loop primers。在LAMP進行時， template DNA加入後，加熱至95℃ 5分鐘再置冰上冷卻，加入8 U Bst polymerase，置於65℃反應35分鐘至1小時，80℃ 10分鐘結束反應後，就可進行結果的判讀。以肉眼或濁度計405nm檢測，也可以快速離心，以得到更確切的結果。再者在反應結束後加入高濃度的SYBR Green Ⅰ螢光顯色直接以肉眼判斷。建立篩選建立九孔重要病毒性疾病之檢驗方法，並據此應用在臨床及檢疫用。研發病毒檢測法，建立新疫病資料，以利輸出入水產動物的檢疫工作。持續研究九孔&#30129;疹病毒，包括病毒特性、致病機制及疫病控制的相關研究。 <br> Abstract: Abalone herpes-like virus has been reported to infection cultured abalone in Taiwan and Australia in the past years. The diagnosis of this disease by PCR and real time PCR has been developed. However, these methods are time consuming, laborious and sometimes non-specific. Loop-mediated isothermal amplification (LAMP) is a rapid, sensitive, inexpensive and powerful tool in applying in different pathogens including fish and shellfish pathogens such as White spot syndrome virus, Taura syndrome virus, Flavobacterium columnare, Spring viraemia of carp virus and Cyprinid herpesvirus-3. In this study, this technique will be study to develop a specific diagnostic protocol for detection of abalone herpes-like virus in the field and for quarantine purpose.恆溫環形核酸增幅法九孔&#30129疹病毒LAMPabaloneherpes-like virus