2019-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/711037摘要：在動物&#32454;胞中，麩氨酸受體ionotropic glutamate receptor (iGluR)扮演著神經訊號 傳導重要作用。在阿拉伯芥中(Arabidopsis thaliana) 被發現至少20 個麩氨酸受體 glutamate receptor-like(GLR)的基因。本研究室之前發現阿拉伯芥麩氨酸受體 GLR3.6參與主根及側根之生長發育，麩氨酸受體GLR3.6透過影響生長素之極向運 輸，間接影響細胞週期基因之表現，進而影響根之生長發育。我們先前維陣列分析 結果顯示麩氨酸受體GLR3.7可能參與非生物性逆境反應。我們假設其他第三群麩 氨酸受體可能參與鹽分及離層酸之反應。本研究中我們將使用阿拉伯芥為模型系&#32479; 。我們將篩選GLR3.7之T-DNA 插入突變株和建立過分表達轉殖株，並觀察其在鹽 分及離層酸處理下根部的外表型。我們將使用鈣離子呈像工具和通道阻礙劑，調查 GLR3.7之T-DNA 插入突變株和過分表達轉殖株中鈣離子之訊號傳導。此外，以為 啟動子之報導基因β-glucuronidase (GUS)將被引進使用，以檢測GLR3.7之T-DNA 插入突變株和過分表達轉殖株中的植物生長素分布。另外，我們將使用微陣列分析 ，找出麩氨酸受體GLR3.7下游受調控之標的基因。特&#21035;地，鈣離子結合蛋白將是 我們的研究焦點。我們也將探尋下游標的基因，此標的基因會接收傳來之鈣離子訊 號，並傳遞訊號至下游以調控根之生長發育。最終，我們希望闡明阿拉伯芥麩氨酸 受體GLR3.7如何參與根生長發育之鈣離子訊號傳導路徑。在長期目標中，我們希 望將該系統引入作物（如水稻）以抵抗鹽逆境並提高作物產量。<br> Abstract: In animal cells, the glutamate receptor ionotropic glutamate receptor (iGluR) plays an important role in neuronal signaling. At least 20 glutamate receptor-like (GLR) genes were found in Arabidopsis thaliana. Our laboratory has previously found that Arabidopsis glutamate receptor GLR3.6 is involved in the growth and development of primary roots and lateral roots. Glutamate receptor GLR3.6 affects the calcium transport, which indirectly affects the expression of cell cycle genes, which in turn affects the growth and development of roots. Our previous microarray analysis result showed that glutamate receptor GLR3.7 may be involved in abiotic stress response. We hypothesized that GLR3.7 is involved in the reaction of salt stress and abscisic acid (ABA) responses. In this study we will use Arabidopsis as our model system. We will screen for T-DNA insertion mutant lines of GLR3.7, and generate GLR3.7 overexpression lines for root phenotyping under salt and ABA-treated condition. We will use calcium ion imaging tools and channel blockers to investigate the signal transduction of calcium ions in T-DNA insertion mutants and overexpressing plants. In addition, the DR5 promoter driven β-glucuronidase (GUS) will be introduced to detect the auxin distribution. In addition, we will use microarray analysis to identify genes that are downstream regulated by GLR3.7. In particular, calcium ion binding proteins will be the focus of our research. By this, we will identify the downstream target gene, which will receive the transmitted calcium ion signal and send the signal downstream to regulate growth and development of the roots. Eventually, we hope to clarify how Arabidopsis glutamate receptor is involved in the calcium signaling pathway of root growth and development. In long term goal, we hope to introduce the system to crops (i.e. rice) against salt stress and improve crop yield.鹽離層酸麩氨酸受體鈣離子根saltabscisic acidglutamate receptorcalciumroot