郭欽明2006-08-312018-07-062006-08-312018-07-061999-07-31http://ntur.lib.ntu.edu.tw//handle/246246/29806吳郭魚為台灣重要養殖魚種之一，惟 因吳郭魚為熱帶魚種，適於高溫養殖，每 在冬季寒流來襲之際，因其耐寒性欠佳而 大量凍斃，有鑑於此，就吳郭魚對低溫之 生理反應與能量代謝的調節進行全盤性研 究，探討本省主要養殖經濟魚種吳郭魚在 低溫適應下能量代謝型態及途徑，為本研 究之主要目標。馴化於25 ℃下的吳郭 魚，分別以不同的低溫25 ℃、20 ℃、15 ℃、10 ℃進行刺激，探討其在低溫壓迫下 能量利用情形，包括溫度耐受性、耗氧量、 24 小時內的血液葡萄糖以及乳酸之時程變 化。吳郭魚之耐寒性欠佳，在15 ℃低溫之 急速刺激下，血糖量的增加非常明顯，僅 僅在6hr 內即由63.91 mg/dL 遽增至132.04 mg/dL，增加幅度達2 倍之多。吳郭魚暴露 於10 ℃ 水溫中， 血糖量僅由41.74 mg/dL，在1 小時內增加到57.15 mg/dL， 雖然血糖量增加幅度不甚顯著，主要原因 在於吳郭魚對10 ℃之刺激反應，生理已失 去調節能力, 呈現衰減的結果。血漿中乳酸 變動的情形，移入溫度為15 ℃者，隨著移 入時間的延長，乳酸濃度有呈現下降的趨 勢，由開始血乳酸濃度16.54 ±0.92 mg/dL 下降至24 hr 的12.11 ±0.28 mg/dL，而移 入溫度為10 ℃之個體，濃度移入後30 分 鐘內仍維持在16.54 ±0.92 mg/dL，但在移 入後一小時，呈現衰減之現象，乳酸量則 下降至9.47 ±2.24 mg/dL，隨即死亡。至 於氧氣消耗量之下降、是與水溫之高低及 剌激持續時間呈相關性。Tilapia, an economically important cultured teleost in Taiwan, has played a significant role in the fisheries development in the past. As a tropical species, they are suitable for culture in the warm waters. However, mass mortality of these cultured tilapia resulted from less tolerable attributes of this species to the cold, has often caused tremendous loss for the tilapia culture industry, when the severe cold current approaches in the winter season. This study is therefore aimed to comprehend the physiological responses of tilapias under the cold shock. The acclimated tilapias at 25 °C were rapidly exposed to varying temperatures of 25 ℃ , 20 ℃, 15 ℃ and 10 ℃, and the physiological responses were monitored under various cold shocks. The parameters monitored included temperature tolerance, oxygen consumption, time-course changes in plasma glucose and lactate. Remarkable hyperglycemic responses were detected in tilapias under the cold shock at 15 ℃, the plasma glucose rapidly increased from 63.91 mg/dl to 132.04 mg/dl in 6 hr, about two-fold increase over the original level. However, a slight elevation in plasma glucose, from 41.74 mg/dl to 57.15 mg/dl in 1 hr, was observed, when the tilapias were exposed acutely to 10 ℃ . The hyperglycemic response at this lethal temperature was found less pronounced as compared to the previous. The observations suggested that the tilapias under 10 ℃ cold shock were unable to perform physiological regulation and compensation properly. The stress responses and the exhaustion of regulatory capability were indicated. With respect to the lactocemic responses under 15 ℃ , the plasma lactate concentration depressed gradually from the initiation of cold shock treatment, decreased from 16.54±0.92 g/dl down to 12.11±0.28 mg/dl in 24 hr. At the extreme temperature of 10 ℃, the plasma lactate maintained at the constant level for 30 min, followed by a notable depression down to 9.47±2.24 mg/dl at the end of 1 hr, and eventually physical exhaustion and death . The changes in the oxygen consumption, which is a reliable biological indicator for the stress responses, was found highly correlated with the temperatures employed and sustained duration of cold shock.application/pdf1248918 bytesapplication/pdfzh-TW國立臺灣大學漁業科學研究所吳郭魚生理反應低溫壓迫TilapiaPhysiological responsesCold shock[SDGs]SDG13[SDGs]SDG14journal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/29806/1/882311B002013B28.pdf