國立臺灣大學醫學院外科王水深2006-08-312018-07-112006-08-312018-07-112000-07-31http://ntur.lib.ntu.edu.tw//handle/246246/29851我們使用光化學反應方法將不同濃度 的Gly-Arg-Gly-Asp (GRGD) peptide接枝於 PU-PEG 材料上，經過ATR-FTIR 分析測得 其皆具有GRGD-peptide 之特性吸收鋒；同 時由ESCA 分析也發現，其表面的氮元素 （N1s）有明顯的增加。透過SEM 觀察 PU-PEG-GRGD 材料表面發現，血漿在室 溫下靜置30 分鐘之後，表面有許多血小板 黏著，且大多數的血小板已活化變形並有 偽足的現象發生。在動態測試中，在25℃ 下，設定剪切率為1290S-1 並作用3 分鐘， 發現血小板黏著的個數雖較靜置的少，但 其活化情形較為顯著，且聚集成較大的團 塊。另外，以此材料做內皮細胞貼附試驗 發現，內皮細胞的貼附率隨著GRGD 接枝 濃度增加而增加。We tested blood compatibility on the modified polyurethane (PU) surface in static and dynamic flow conditions. We had been succeeding to graft glycol-Gly-Arg-Gly-Asp (GRGD) peptide on the PU surface by photochemical reaction. To characterize the PUPEG- GRGD, ATR-FTIR and ESCA analysis were applied to confirm this result. ESCA analysis showed that the surface of PU-PEGGRGD increased the its component of N1s atom. In addition, PU-PEG-GRGD surface showed the adsorption peak on FTIR absorption analysis, which might indicate GRGD peptide being grafted. We did grafted different concentration of GRGD to PU-PEG surfaces, and found that the surfaces can facilitate endothelia cell adhesion and growth with the increasing of the concentration of GRGD.application/pdf971148 bytesapplication/pdfzh-TW國立臺灣大學醫學院外科blood compatibilityPUGRGDphoto- chemical reactionESCAendothelia cell光化學反應動態測試內皮細胞reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/29851/1/892320B002148M08.pdf