2002-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/677642摘要：植物生長發育的每個階段都受到光的調控，尤其幼苗的生長對光特別敏感。但是，光並不是單獨作用；乃是與植物荷爾蒙共同合作以調節植物的光型態發生。近來對於光型態發生的研究暗示auxin、gibberellin(GA) 和brassinosteroid(BR)也參與了下胚軸(hypocotyls)伸長的過程。 利用chromosome walking的方法，FIN219基因最近已被分離，它扮演著光敏素A與下游的光調控者COP1之間的橋樑，並且 encode一個575 胺基酸的GH3-like蛋白質。GH3的基因首先在大豆中被分離出來，它可受到auxin的誘導，五分鐘內即可產生出來；有趣的是，在阿拉伯芥的基因體中存在著至少有20個GH3-like的基因，它們之間在胺基酸的次序上有35%-47%的相同性，同時也存在著一些很保留(conserved)的區域，但是它們的功能尚不清楚。FIN219是COP1的一個抑制者(suppressor)，並且是光敏素A訊息傳遞中的上游成員。為了進一步的了解這些GH3-like基因的功能以及在植物生長發育過程中所扮演的角色，本計劃乃是要分離出所有這些基因，並且研究它們<br> Abstract: Light regulates almost every aspect of plant growth and developmental processes, among which seedling development is most sensitive to light conditions. However, light does not function independently. It is integrated with endogenous plant hormones for coordinated regulation of plant photomorphogenesis. Recent studies on photomorphogenesis suggest that auxins, gibberellins(GA) and brassinosteroids(BR) are involved in the process of hypocotyl elongation. FIN219, defining a link between phytochrome A and the downstream regulator COP1 in light control of Arabidopsis development, has been cloned lately by map-based method and encodes a GH3-like protein with 575 amino acids. GH3 genes were first cloned in soybeans and induced rapidly within 5 minutes by exogenous auxin. Intriguingly, there are at least 20 GH3-like genes present in Arabidopsis genome. Sequence comparison indicates that there is 35%-47% identity in the level of amino acid sequences among them, in which some conserved regions are also found. However, their functions remain unknown. FIN219 functions as a suppressor of COP1 and also acts as a component upstream phytochrome A-mediated signal transduction pathway in Arabidopsis. To further understand the functions of these GH3-like genes and possible roles during plant developmental processes, the project is going to isolate all of these genes and investigate the relationship between them and FIN219, and their involvement in light signaling pathways.GH3-like 基因光敏素A光型態發生FIN219COP1GH3-like genesphytochrome AphotomorphogenesisFIN219COP1