Chang, Jung-HsuanJung-HsuanChangChou, Chia-HuaChia-HuaChouJUI-CHING WULiao, Keng-MaoKeng-MaoLiaoLuo, Wei-JiaWei-JiaLuoHsu, Wei-LunWei-LunHsuChen, Xuan-RenXuan-RenChenSUNG-LIANG YUSZU-HUA PANPAN-CHYR YANGKANG-YI SU2023-05-252023-05-252023-04-102399-3642https://scholars.lib.ntu.edu.tw/handle/123456789/631364Long-form collapsin response mediator protein-1 (LCRMP-1) belongs to the CRMP family which comprises brain-enriched proteins responsible for axon guidance. However, its role in spermatogenesis remains unclear. Here we find that LCRMP-1 is abundantly expressed in the testis. To characterize its physiological function, we generate LCRMP-1-deficient mice (Lcrmp-1-/-). These mice exhibit aberrant spermiation with apoptotic spermatids, oligospermia, and accumulation of immature testicular cells, contributing to reduced fertility. In the seminiferous epithelial cycle, LCRMP-1 expression pattern varies in a stage-dependent manner. LCRMP-1 is highly expressed in spermatids during spermatogenesis and especially localized to the spermiation machinery during spermiation. Mechanistically, LCRMP-1 deficiency causes disorganized F-actin due to unbalanced signaling of F-actin dynamics through upregulated PI3K-Akt-mTOR signaling. In conclusion, LCRMP-1 maintains spermatogenesis homeostasis by modulating cytoskeleton remodeling for spermatozoa release.en[SDGs]SDG3journal article10.1038/s42003-023-04778-2370379962-s2.0-85152117536https://api.elsevier.com/content/abstract/scopus_id/85152117536