JENNY LING-YU CHENTsai Y.-C.Tsai M.-H.Lee S.-Y.Wei M.-F.SUNG-HSIN KUOMING-JIUM SHIEH2021-04-302021-04-3020170934-0866https://scholars.lib.ntu.edu.tw/handle/123456789/558397Resistance of cancer stem cells to radiotherapy remains a major obstacle to successful cancer management. Prominin-1 (PROM1) is a cancer stem cell marker. Nanoparticle (NP) chemotherapeutics preferentially accumulate in tumors and are able to target cancer and cancer stem-like cells through cancer cell-specific ligands, making them uniquely suited as radiosensitizers for chemoradiation therapy. Using a biocompatible apoferritin NP, a PROM1-targeted NP carrying irinotecan (PROM1-NP) is engineered. The synergistic effect of the NP and irradiation is evaluated in PROM1-overexpressing HCT-116 colorectal cancer cell lines in vitro and in vivo. PROM1-NP has a size of 17.2 ± 0.2 nm and surface charge of ?13.5 ± 0.2 mV. It demonstrates higher intracellular uptake than nontargeted NP or irinotecan alone. Treatment with PROM1-NPs decreases HCT-116 cell proliferation in a dose- and time-dependent manner. In vitro radiosensitization reveals that PROM1-NP is significantly more effective as a radiosensitizer than nontargeted NP or irinotecan. HCT-116 tumor xenograft growth is markedly slower following treatment with PROM1-NP plus irradiation, suggesting that PROM1-NP is more effective as a radiosensitizer than irinotecan and nontargeted NP in vivo. This study provides the first preclinical evidence of the effectiveness of PROM1-targeted NP formulation of irinotecan as a radiosensitizer. ? 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim[SDGs]SDG3Biocompatibility; Cell culture; Cell proliferation; Cells; Cytology; Irradiation; Nanoparticles; Stem cells; Tumors; Apoferritin; Chemoradiation therapies; Colorectal cancer; Colorectal cancer cell; Intracellular uptake; Irinotecan; Radio-sensitization; Synergistic effect; Diseasesjournal article10.1002/ppsc.2016004242-s2.0-85014077314